利用SSR标记鉴定杂交粳稻‘常优1号’种子纯度

Identification of Seed Purity on Hybrid Japonica Rice‘Changyou 1'Using SSR Markers

为了建立一套快速有效的鉴定交粳稻‘常优1号’种子纯度的方法,本研究利用SSR分子标记技术,对三系杂交粳稻‘常优1号’及其父母本的多态性进行了引物筛选和种子纯度鉴定研究。从24对SSR引物中筛选到1对在‘常优1号’和其双亲之间表现为稳定的共显性且能将杂交种及其亲本完全区分开的引物。利用筛选的RM208引物对‘常优1号’200粒种子进行鉴定,结果表明,‘常优1号’种子纯度为96%,与大田1 000株杂交种纯度鉴定结果 98.2%相比,SSR标记与大田种植鉴定结果基本一致。研究结果表明,引物RM208可用于‘常优1号’种子纯度的鉴定,有助于‘常优1号’的推广应用。

In order to establish a rapid and effective method to identify the purity of hybrid japonica rice ＇Chang- you 1 ＇, the polymorphism of three-line hybrid japonica rice ＇Changyou 1＇ and its parents were detected with simple sequence repeat （SSR） markers. In this study, 24 pairs of SSR primers were screened, and 1 of them was able to present stable co-dominance and to distinguish the hybrid from its parents effectively. 200 individual seeds of ＇Changyou 1＇ were tested with RM208, and the results showed that the seed purity was 96%, which was closed to the results validated in the field （98.2%）. This study indicated SSR primer RM208 could be used to identify the purity of hybrid japonica rice Changyou 1 ＇, and to facilitate this hybrid applying in rice production as well.