木薯细胞壁酸性转化酶基因MeCWINV1启动子的克隆及其在烟草中的瞬时表达分析

Cloning of Me CWINV1 Promoter from Cassava and Its Transient Expression Analysis in Tobacco

为了解木薯细胞壁酸性转化酶基因Me CWINV1胁迫诱导模式及调控机制,本研究利用PCR的方法,从木薯基因组中分离到一段Me CWINV1基因启动子序列(Gen Bank登录号:KC465190)。分析结果显示,该启动子长度865 bp,含有TATA box和CAAT box保守元件,以及MBS、HSE、AT1等多个与植物逆境胁迫相关的元件。通过构建GUS基因融合表达载体,对该启动子在烟草中的瞬时表达进行分析。结果表明,GUS基因主要在烟草叶脉中表达。研究结果为进一步研究该启动子的功能奠定了基础。

In order to study the inducement pattern and regulating mechanism of MeCIVINVl in cassava, we isolated an 865 bp promoter region upstream of the MeCWINV1 gene （GenBank accession No. KC465190） from cassava （Manihot esculenta） genomic DNA using PCR methods. Sequence analysis found that it contains a typical TATA box and CAAT box, and several cis-acting elements that relate to plant stress responses, such as MBS, HSE, and AT1 transcription factor. Furthermore, transient expression in tobacco was analyzed by constructing a promoter： GUS fusion expressing vector. The results showed that the GUS gene mainly expressed in tobacco veins. This will be a basis for further investigating the function of the MeCIVINV1 gene promoter.