兔门静脉种植VX2癌栓CT灌注参数与血管内皮生长因子的相关性

Relationship Between CT Perfusion Parameters and Vascular Endothelial Growth Factor Expression in Rabbits Portal Vein VX2 Implanting Tumor Emboli

目的建立兔VX2肿瘤模型,采用多层螺旋CT(MSCT)灌注成像技术探讨兔门静脉VX2种植癌栓灌注参数与血管内皮生长因子(VEGF)表达的相关性。材料与方法 8只实验兔经门静脉内接种VX2肿瘤,成瘤后行MSCT灌注扫描,测量并比较门静脉癌栓、近瘤灶和远离瘤灶肝脏的肝血流灌注量(HBF)、肝血容积(HBV)、毛细血管表面通透性(PS)及平均通过时间(MTT);摘取门静脉移植VX2癌栓,采用免疫组化检测癌栓组织VEGF表达,分析肝脏灌注参数与VEGF的相关性。结果近瘤灶和远离瘤灶肝脏各CT灌注参数间差异无统计学意义(P>0.05);门静脉癌栓区HBF、HBV及PS均较近瘤灶区和远离瘤灶区增高(P<0.05),MTT较近瘤灶区和远离瘤灶区降低(P<0.05)。门静脉VX2种植癌栓区HBF、HBV、PS与VEGF表达均呈正相关(r=0.711、0.646、0.626,P<0.05),MTT与VEGF表达呈负相关(r=-0.565,P<0.05)。结论兔门静脉VX2种植癌栓MSCT灌注参数与VEGF表达具有相关性,MSCT能够评价门静脉种植VX2癌栓的血管生成。

Purpose To establish rabbit VX2 tumor model and to explore the relation between perfusion parameters and the expression of the VEGF in the portal vein VX2 implanting tumor emboli. Materials and Methods VX2 tumor was implanted in the portal vein of eight experimental rabbits. Multi-slice CT （MSCT） perfusion scan was performed after tumor formation to measure and compare portal vein tumor thrombus, hepatic blood lfow （HBF） near tumor foci and far away from tumor foci, hepatic blood volume （HBV）, probability of surface area product （PS） and mean transit time （MTT）. The VX2 tumor emboli were then resected to analyze the relationship between the liver perfusion parameters and VEGF expression using immunohistochemical method. Results MSCT liver perfusion parameters were not statistically signiifcant between foci close to or far away from the tumor （P＆gt;0.05）. The HBF, HBV and PS within the tumor emboli were higher than that in hepatic parenchyma （P〈0.05） and the MTT was higher （P〈0.05）. There was positive correlation （r=0.711, 0.646 and 0.626, P〈0.05） between the HBF, HBV and PS of portal vein VX2 tumor emboli and VEGF expression, and there was negative correlation between MTT and VEGF expression （r=-0.565, P〈0.05）. Conclusion MSCT perfusion parameters in the portal vein VX2 implanting tumor emboli and the expression of VEGF are positively related. MSCT can evaluate the angiogenesis of portal vein VX2 implanting tumor emboli.