科研型肝癌肝移植临床标本库的建立及意义

Establishment and significance of research-based clinical liver transplantation specimen bank for hepatocellular carcinoma

背景:建立规范化的肝癌肝移植临床样本库可为开展相关领域研究提供资源平台,是进行科学研究的先决条件。目的:初步建立科研型肝癌肝移植临床标本库,研究并制定规范化的肝癌肝移植标本采集、处理、保存操作流程,建立完善、全面的肝癌肝癌肝移植标本信息化管理系统。方法:鉴国内外标本库建立的标准化程序,制定肝癌肝移植标本库操作流程及质量控制体系,规范化收集、处理及保存肝癌肝移植受者的肿瘤组织及血液标本,同时收集良性肝病肝移植受者及健康供者的肝组织及血液标本作为研究对照。对所收集的标本及相关临床资料信息进行系统化管理。结果与结论:自2009年8月肝癌肝移植标本库收集有完整临床资料的501例肝移植供、受者组织及血液标本,其中肝癌标本203例,良性肝病标本214例,健康供肝标本84例。肝癌标本包括肿瘤组织、癌旁组织及远端非癌组织标本,共计1 773份;随机抽取45例标本进行质量监测,显示所收集的标本质量较好,开发了规范化的标本信息资料计算机管理系统。实验初步建立了具有一定规模的科研型肝癌肝移植标本库,有利于提高标本的质量,可操作性较好。

BACKGROUND：The establishment of a standardized clinical liver transplantation specimen bank is the primary condition for scientific research in this field, which can help to provide a qualified sample resource platform for research. OBJECTIVE：To primarily establish biological specimen bank of hepatocelular carcinoma for liver transplantation, to explore the standardized procedures of specimen colection, processing and preservation of hepatocelular carcinoma for liver transplantation, and to establish the sound and comprehensive information management system of clinical information of colected specimens. METHODS： In accordance with standardized procedures to establish biological specimen banks, the operational processes and quality control system were formulated. Liver tissue and blood samples of hepatocelular carcinoma recipients undergoing liver transplantation were regularly colected, managed and stored. Simultaneously, liver tissue and blood samples of benign liver disease in liver transplant recipients and of healthy donor were colected as controls. A systematic management was conducted in colected specimens and＆amp;nbsp;corresponding clinical information. RESULTS AND CONCLUSION：From August 2009, tissue and blood samples of 501 cases of receipts and donors undergoing liver transplantation with complete clinical information were colected from the specimen bank, including 203 hepatocelular carcinoma specimens, 214 benign liver disease specimens and 84 healthy donor specimens. These specimens included tumor tissue, adjacent tissues and distal non-cancerous tissue specimens, totaly 1 773. A total of 45 specimens were randomly selected for quality monitoring. The colected specimens had a high quality. Specimen information data computer management system was developed. This study initialy established a standardized research-based clinical transplantation specimen bank, which is helpful to elevate sample quality and has a good manipuility.