HPVL1在不同宫颈病变组织中的表达规律探讨

Expression pattern of HPV L1 in different cervical lesion tissues

目的探讨影响HPV L1蛋白在肿瘤组织内表达的因素,寻找HPV L1蛋白在不同宫颈病变中的表达规律。方法收集不同宫颈病变组织,检测标本内HPV16和HPV18基因分型及基因组物理状态;用ELISA、蛋白质印迹和免疫组织化学方法检测标本内HPV L1的表达情况。结果共收集到48例标本,其中42例HPV16阳性,3例HPV18阳性。HPV16阳性标本中,25例病毒基因组为游离型,7例为混合型,10例为整合型;HPV18阳性标本全部为整合型。ELISA及蛋白质印迹检测显示,随着病变程度的加重,HPV L1的表达量有逐渐降低的趋势。免疫组织化学显示,组织内的HPV L1阳性反应有多种形式,依病变组织内病毒基因组物理状态、病变组织分化程度以及病变进程的不同而不同。结论 HPV L1在宫颈病变组织内的表达有一定的结构基础,其表达量与病毒基因组在病变组织和细胞内的物理状态、肿瘤组织的分化程度、病毒基因组插入宿主基因组基因序列的状态有着非常密切的关系。

Objective To explore the factors influencing the HPV L1 protein expression in tumor tissues and to understand the expression pattern of HPV L1 protein in different cervical lesion tissues. Methods Different cervical lesion tissues were collected to detect genotypes and genome physical states of HPV 16 and 18. ELISA, Western blotting analysis, and immunohistochemical staining were used to examine the expression of HPV L1 protein in the tissues. Results A total of 48 specimens were collected, with 42 being HPV16 positive and 3 being HPV18 positive. In HPV16 positive specimens, 25 harbored exclusively the episomal form, 7 carried mixed forms, and 10 had integrated type. All the HPV18 positive specimens belonged to the integrated type. The results of ELISA and Western blotting analysis displayed that HPV L1 expression decreased with the aggravation of the lesion severities. The immunohistochemistry findings showed that L1 positive reactions were of different forms and varied with different physical states of viral genome, differentiation degrees, and progression of lesion tissues. Conclusion HPV L1 expression in cervical lesion tissues has its structural foundation, and the expression levels are closely related to many factors, including the physical state of virus genome in lesion tissues, differentiation degrees of tumor tissue, and the state of virus gene sequence being inserted into host ones.