电针对大鼠穴区血管内皮细胞间黏附因子1表达及肥大细胞分布的影响

Effects of Electroacupuncture Stimulation of“Zusanli”(ST 36)on the Regional Vascular Intercellular Adhesion Molecule-1mRNA Expression and Mast Cell Activity in Normal Rats

目的:观察电针后大鼠皮下组织血管内皮细胞间黏附因子1(intercellular adhesion molecule-1,ICAM-1)表达及肥大细胞(mast cell,MC)分布,探讨电针刺激后穴区血管ICAM-1mRNA表达的变化,及其与MC活性的关系。方法:将20只大鼠随机均分为正常组和电针组,电针组给予双侧"足三里"穴电针刺激25min。用原位杂交技术观察两组大鼠"足三里"穴区皮下组织ICAM-1mRNA的表达;同时用乙酰胆碱酯酶组化染色并甲苯胺蓝复染法观察"足三里"穴区皮下组织MC的分布。结果:电针组"足三里"穴区组织ICAM-1mRNA阳性表达明显高于正常组(P<0.01);电针组穴区组织MC数和MC脱颗粒率均升高,与正常组比较均具有统计学意义(P<0.01)。结论:电针可增强正常大鼠穴区组织血管内皮ICAM-1mRNA的表达,ICAM-1对电针促进肥大细胞向穴区迁移、聚集具有一定趋化作用。

Objective To observe the effect of electroacupuncture （EA） stimulation of ＂Zusanli＂ （ST 36） on intercellu- lar adhesion molecule-1 （lOAM-l） mRNA expression and mast cell （MC） distribution in the stimulated subcutaneous tissues in nor- mal rats, so as to study the correlation between lOAM-1 mRNA expression and MC activity during EA. Methods Twenty Wistar rats were randomly assigned to normal group and EA group. EA（2 Hz/15 Hz, 0. 1 mA） stimulation was applied to the bilateral ＂Zusanli＂ （ST 36） for 25 min. The expression of lOAM-1 mRNA in subcutaneous tissues of the left ＂Zusanli＂ （ST 36） area was de- tected by in situ hybridization, and MC activity of the same tissues was observed under microscope after staining with acetylcho- line esterase （ACHE） and toluidine blue （TB,counterstaining）, respectively. Results Compared with the normal group, the ICAM-1 mRNA expressed at a markedly higher level in the subcutaneous tissues of the ＂Zusanli＂ （ST 36） region in the EA group （P〈0.01 ）, and MC counts were also significantly increased in the EA group （P〈0.01）. Conclusion EA stimulation up-regu- lates the expression of lOAM-1 mRNA and enhances MC release from the stimulated regional subcutaneous tissue in healthy rats, suggesting lOAM-1 played an important role in affecting the movement and recruitment of MCs towards acupoint during EA.