盐制对杜仲治疗去卵巢大鼠骨质疏松症影响的研究

Study of the effect of salt-roast processing product of Eucommia ulmoides Oliv.on osteoporosis in ovariectomized rats

目的研究杜仲及其盐制品对去卵巢大鼠骨质疏松症的治疗作用,并探讨盐制对其治疗作用的影响。方法 40只SD雌性大鼠分为伪手术组、模型对照组、杜仲生品治疗组和杜仲盐制品治疗组,除假手术组施行假手术外,其余均行手术彻底摘除卵巢,术后4周开始杜仲生品及盐制品水提液灌胃给药(4.0 g/kg·d),实验过程称体质量,连续给药12周后,颈动脉取血,测定血钙(S-Ca)、血磷(S-P)含量,ELISA试剂盒双抗体夹心法测定血清碱性磷酸酶(ALP)、骨钙素(OCN)、雌二醇(E2)含量;动物处死后剥取完整子宫称重,采用HE染色法进行子宫病理学检查,剥离双侧股骨,双能X射线衍射法测定大鼠股骨骨密度。HE染色法观察股骨骨小梁形态和成骨细胞数量,micro-CT扫描观察股骨骨小梁微体系结构。结果杜仲生品及盐制品能有效抑制去卵巢所致的大鼠体重增加,升高血清钙、雌二醇的含量,降低血磷、碱性磷酸酶和骨钙素的含量。增加大鼠股骨骨密度,改善股骨骨小梁微体系结构,增加股骨骨小梁成骨细胞数量,且长期用药对子宫无明显刺激作用。结论杜仲生品及盐制品对于大鼠去卵巢所引起的骨质疏松症有良好治疗作用,且盐制品效果优于生品。

Objective To evaluate the anti-osteoporosis effect of Eucommia ulmoides Oliv.and the effect of salt-roast processing product on the treatment efficacy in ovariectomized rats.Methods Forty female SD rats were divided into Sham group, OVX group, Eucommia ulmoides Oliv.group, and salt-roast processing Eucommia ulmoides Oliv.group.All the rats except in Sham group were ovariectomized.After 4 weeks of the operation, rats received oral Eucommia ulmoides Oliv.or its salt-roast processing product （4.0 g/kg？d） daily.The body weight of the rats was recorded.After a 12-week treatment, the serum calcium （S-Ca） and phosphorus （ S-P） were measured.Serum ALP, osteocalcin （ OCN） , and estrogen （ E2 ） were determined using ELISA kits.The uterus was weighed after execution of the rats and pathological examination was performed using HE staining.Bone mineral density of the bilateral femur was tested using dual-energy X-ray absorptiometry.Morphology of the trabecular and the number of the osteoblast were observed using HE staining.Microarchitecture of the trabecular bone was observed using micro-CT scan.Results Oral administration of Eucommia ulmoides Oliv.and the salt-roast processing product effectively inhibited ovariectomy-induced the body weight increase, increased S-Ca and E2 , decreased S-P, ALP, and OCN.They increased BMD of the femur, improved microarchitecture of the trabecular bone, and increased the number the osteoblast.The long-term use of the drug had no stimulating effect on the uterus.Conclusion Eucommia ulmoides Oliv.and the salt-roast processing product have good therapeutic effect on ovariectomy-induced osteoporosis in rats, and the effect of salt-roast processing product is better than unroasted drug.