血细胞分离机AutoPBSC程序与MNC程序采集外周血干细胞的效果比较及影响因素分析

Analysis of the Efficiency and Influence Factors of PBSC Collection with AutoPBSC and MNC Procedure of Cell Separator

本研究旨在分析COBE Spectra血细胞分离机自动采集程序(Auto PBSC程序)与半自动采集程序(MNC程序)在外周血干细胞采集中的效果及影响因素。109例采集按对象不同分为自体患者组(患者)和异基因供者组(供者),通过对采集物中干细胞数量与质量及采集程序特点的比较,对两种采集程序在两组中的采集结果及影响因素分别进行了分析。结果表明,在全血处理量基本相同的情况下,患者组和供者组两种程序采集物中MNC%与CD34+%、CD34+细胞数及血红蛋白含量差异无显著性(P>0.05);与MNC程序比较,Auto PBSC程序采集物中血小板混入少,采集物体积小,但抗凝剂用量多,采集时间长(P<0.05)。相关性分析显示,患者组两种程序采集物中MNC数(r=0.314,P=0.015)、CD34+细胞数(r=0.922,P=0.000)与采集前对应参数呈正相关,采集物中CD34+细胞数与WBC数(r=0.369,P=0.004)及MNC数(r=0.495,P=0.000)呈正相关;供者组Auto PBSC程序采集物中MNC数与采集前呈正相关(r=0.896,P=0.000),MNC程序采集物中CD34+细胞数与采集前呈正相关(r=0.666,P=0.000)。患者组Auto PBSC程序采集物中MNC数与CD34+细胞数在男性显著高于女性(P<0.05),在年龄40岁以下的患者显著高于年龄在40岁以上的患者(P<0.05),而MNC程序年龄在40岁以上患者采集的CD34+细胞数显著高于年龄40岁以下患者(P<0.05)。供者组仅MNC程序采集物中MNC数与CD34+细胞数在男性显著高于女性(P<0.05)。结论:在全血处理量相同时,自体患者和异基因供者PBSC采集中两种程序收获的MNC纯度与CD34+细胞纯度及浓度高度一致,但自体患者PBSC采集结果受年龄与性别影响较大。

This study was aimed to analyze the efficiency and influence factors of PBSC collection by an automatic（ AutoPBSC procedure）and a semiautomatic apheresis procedure（ MNC procedure）of COBE Spectra cell separators.According to the different objectsA total of 109 apheresis cases were divided into autologous cohort（ patient）and allogeneic cohort（ donor）.The quantity and quality of the collections and the characteristics of apheresis procedure were comparedthe yields and influence factors of two cohorts with two kinds of procedures were analyzed respectively.The results showed that the collections of two procedure in patients and donors which processed the similar blood volumes were insignificantly different in MNC%CD34^＋ %CD34^＋ cell counts and Hb concentration（ P〉0.05）; the collections by AutoPBSC procedure had got fewer plateletsless product volumes whereas more ACD-A usedlonger apheresis time in comparison with MNC procedure（ P〈0.05）.Correlation analysis indicated that MNC（r = 0.314 P =0.015）,CD34^＋ cell counts（r = 0.922 P = 0.000） in collections were positively correlated with preahperesis in the autologus cohort by two proceduresCD34^＋ cell counts were correlated with WBC（r = 0.369 P = 0.004） and MNC（r= 0.495 P = 0.000） in collectionsMNC（ r = 0.896 P = 0.000） was positive correlated with preahperesis by AutoPBSC procedures and CD34^＋ cell counts alsor = 0.666 P = 0.000 by MNC procedure in the allogeneic cohort.Male had got more MNC and CD34^＋ cell counts than female（ P〈0.05）,age〉40 had got more MNC and CD34^＋ cell counts than age 40（ P〈0.05） in patients by AutoPBSC procedureage 40 had got more CD34^＋ cell counts than age≤40 by MNC procedure（P〈0.05）.Only male had got more MNC and CD34^＋ cell counts than female（ P〈0.05） by MNC procedure in donors.It is concluded that with same amount of blood processingthe PBSC collections from autologous patients and allogeneic donors had got a high degree of uniformly in purity of MNC and purity and concentration of CD34^＋ cell counts by two procedurewhereas sex and age imposed more influence on PBSC collection in autologous.