Cu跨入人红细胞膜的动力学研究

Kinetics of Cu Crossing Human Erythrocyte Membrane

本研究旨在探讨Cu2+跨入人红细胞的几种影响因素,包括Cu2+的浓度,介质的p H值,温育的温度,温育的时间的影响,并导出Cu2+离子跨入人红细胞的动力学方程。采用Cu2+溶液对人血悬浮红细胞温育,消化后利用原子吸收光谱法测定不同Cu2+浓度、温育温度、介质p H、温育时间下Cu2+离子跨入红细胞的含量。结果显示,Cu2+离子跨入红细胞的含量分别随着胞外Cu2+浓度的增大和温育温度的增大而增大。Cu2+离子跨入红细胞的含量在p H 6.2-7.4呈现增大的趋势,p H 7.4时达到最大值,而在p H 7.4-9.2之间呈逐步减小的趋势。结论:Cu2+离子跨入红细胞在120 min内符合一级动力学特点,线性方程为103×Y=0.0497t+6.5992。

This study was aimed to investigate various factors influencing the proceduction of Cu （ II ） crossing human erythrocyte membrane, including concentration of Cu^2＋ , pH value of the medium, temperature and time of incubation, and to derive kinetic equation of Cu （ II ） crossing human erythrocyte membrane. Suspension red blood cells were incubated by Cu^2＋, then content of Cu^2＋ crossed human erythrocyte membrane was determined by atomic absorption spectrometry under various conditions after digestion. The results showed that content of Cu^2＋ crossed human erythrocyte membrane increased with the increase of extracellular Cu^2＋ and enhancement of incubation temperature, and the content of Cu^2＋ crossed human erythrocyte membrane showed a increasing tendency when pH reached to 6.2 - 7.4, and to maximum at pH 7.4, then gradually decreased at range of pH 7.4 - 9.2. It is concluded that the Cu^2＋ crossing human erythrocyte has been confirmed to be the first order kinetics characteristics within 120 min, and the linear equation is 10^3 × Y = 0. 0497t ＋ 6.5992.