期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

MicroRNA-378＊通过抑制CTGF促进人骨髓间充质干细胞凋亡 被引量：3

MicroRNA-378* enhances apoptosis of human mesenchymal stem cells by repressing expression of CTGF
下载PDF
导出
摘要 目的:研究年龄相关microRNA-378*(miR-378*)对人骨髓间充质干细胞(h MSCs)存活和凋亡的调控作用。方法:通过microRNA芯片和qRT-PCR检测供体年龄对h MSCs中miR-378*表达的影响;通过H2O2诱导h MSCs凋亡;通过转染miR-378*模拟物或抑制物,过表达或抑制miR-378*的表达;用MTT、LDH、caspase-3/7、TUNEL检测等方法研究其对h MSCs存活和凋亡的影响;通过siRNA研究结缔组织生长因子(CTGF)对h MSCs存活和凋亡的影响。结果:随供体年龄增加,h MSCs中miR-378*的表达增加。H2O2刺激可促进miR-378*表达,抑制CTGF表达。过表达miR-378*可减少h MSCs的存活,促进细胞凋亡。相反,抑制miR-378*的表达促进h MSCs的存活,减少细胞凋亡。同时抑制miR-378*和CTGF的表达,使miR-378*失去对h MSCs存活和凋亡的调控作用。直接抑制CTGF的表达可减少h MSCs的存活,促进细胞凋亡。结论:miR-378*通过抑制CTGF的表达减少h MSCs存活,促进h MSCs凋亡。 AIM： To investigate the effects of microRNA-378 ＊ （ miR-378 ＊ ） on the survival and apoptosis of human mesenchymal stem cells （hMSCs）. METHODS： The expression of miR-378 ＊ was determined by microRNA arrays and quantitative real-time PCR （qRT-PCR）. H202 was used to induce hMSCs apoptosis. By transfection of miR- 378 ＊ mimic or inhibitor, we up-regulated or down-regulated miR-378 ＊ expression in hMSCs. The effect of miR-378 ＊ and connective tissue growth factor （CTGF） on hMSC survival and apoptosis were detected by MTI＇, LDH, caspase-3/7 and TUNEL assays. RESULTS： The expression of miR-378 ＊ was up-regulated in the old hMSCs compared with the young hMSCs. H202 increased the expression of miR-378 ＊ , decreased the expression of CTGF. Up-regulation of miR-378 ＊ re- suited in increasing apoptosis and decreasing survival of hMSCs. Conversely, down-regulation of miR-378 ＊ resulted in de- creasing cell apoptosis and increasing survival. The regulation of miR-378 ＊ on hMSC apoptosis and survival was attenuated by inhibiting the expression of miR-378 ＊ and CTGF together. Direct repression of CTGF expression inhibited the hMSC survival and increased apoptosis. CONCLUSION： miR-378 ＊ enhances apoptosis of hMSCs by repressing the expression of CTGF.
作者 董珺 曾波航 刘宁宁 莫沛 熊龙根 刘世明 黎佼
机构地区 广州医科大学附属第二医院肿瘤科 广州医科大学附属第二医院广州心血管疾病研究所 广州医科大学附属第二医院心内科
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2014年第12期2238-2242,共5页 Chinese Journal of Pathophysiology
基金 国家自然科学基金青年项目(No.81401156) 广州市属高校科研计划项目(No.2012C232) 广州医科大学博士科研项目(No.2012C42)
关键词 人骨髓间充质干细胞 MicroRNA-378＊ 细胞凋亡 Human mesenchymal stem cells MicroRNA-378 ＊ Apoptosis
分类号 R363 [医药卫生—病理学]
  • 相关文献

参考文献13

  • 1黎佼,陈敏生,杨伟健,张振辉,钟赟,刘世明.年轻骨髓间充质干细胞可通过细胞融合改善年老骨髓间充质干细胞功能[J].中国病理生理杂志,2010,26(5):976-981. 被引量：7
  • 2Li J, Dong J, Zhang ZH, et ah miR-10a restores human mesenchymal stem cell differentiation by repressing KLF4 [J]. J Cell Physiol, 2013, 228(12):2324-2336.
  • 3黎佼,董珺,张振辉,田朝伟,成传访,吴功雄,刘世明.MicroRNA-196a通过HOXB7调控人骨髓间充质干细胞的增殖功能[J].中国病理生理杂志,2014,30(2):278-285. 被引量：7
  • 4Knezevic I, Patel A, Sundaresan NR, et al. A novel car- diomyocyte-enriched microRNA, miR-378, targets insulin- like growth factor 1 receptor: implications in postnatal car- diac remodeling and cell survival[ J ]. J Biol Cbem, 2012, 287 (16) : 12913-12926.
  • 5Li H, Chang L, Du WW, et al. Anti-microRNA-378a en- hances wound healing process by up-regulating integrin be- ta-3 and vimentin [J]. Mol Ther, 2014, 22 (10): 1839-1850.
  • 6Zhang GJ, Zhou H, Xiao HX, et al. MiR-378 is an inde- pendent prognostic factor and inhibits cell growth and inva- sion in colorectal cancer [ J ]. BMC Cancer, 2014, 14: 109.
  • 7Lee DY, Deng Z, Wang CH, et al. MicroRNA-378 pro- motes cell survival, tumor growth, and angiogenesis by targeting SuFu and Fus-1 expression[ J]. Proc Natl Acad Sci U S A, 2007, 104(51) :20350-20355.
  • 8Kim SW, Kim HW, Huang W, et al. Cardiac stem cells with electrical stimulation improve ischaemic heart function through regulation of connective tissue growth factor and miR-378 [ J]. Cardiovasc Res, 2013, 100(2) :241-251.
  • 9Charrier A, Brigstock DR. Regulation of pancreatic func- tion by connective tissue growth factor (CTGF, CCN2) [J]. Cytokine Growth Factor Rev, 2013, 24(1) :59-68.
  • 10Bennewith KL, Huang X, Ham CM, et al. The role of tumor cell-derived connective tissue growth factor (CTGF/ CCN2) in pancreatic tumor growth [ J ]. Cancer Res, 2009, 69 (3) :775-784.

二级参考文献30

  • 1Stolzing A,Scutt A.Age-related impairment of mesenchymal progenitor cell function[J].Aging Cell,2006,5(3):213-224.
  • 2Baxter MA,Wynn RF,Jowitt SN,et al.Study of telomere length reveals rapid aging of human marrow stromal cells following in vitro expansion[J].Stem Cells,2004,22(5):675-682.
  • 3Moerman EJ,Teng K,Lipschitz DA,et al.Aging activates adipogenic and suppresses osteogenic programs in mesenchymal marrow stroma/stem cells:the role of PPAR-gamma 2 transcription factor and TGF-beta/BMP signaling pathways[J].Aging Cell,2004,3(6):379-389.
  • 4Terada N,Hamazaki T,Oka M,et al.Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion[J].Nature,2002,416(6880):542-545.
  • 5Alvarez-Dolado M,Pardal R,Garcia-Verdugo JM,et al.Fusion of bone-marrow-derived cells with Purkinje neurons,cardiomyocytes and hepatocytes[J].Nature,2003,425(6961):968-973.
  • 6Vassilopoulos G,Wang PR,Russell DW.Transplanted bone marrow regenerates liver by cell fusion[J].Nature,2003,422(6934):901-904.
  • 7Allsopp RC,Cheshier S,Weissman IL.Telomerase activation and rejuvenation of telomere length in stimulated T cells derived from serially transplanted hematopoietic stem cells[J].J Exp Med,2002,196(11):1427-1433.
  • 8Ryu BY,Orwig KE,Oatley JM,et al.Effects of aging and niche microenvironment on spermatogonial stem cell self-renewal[J].Stem Cells,2006,24(6):1505-1511.
  • 9Conboy IM,Conboy MJ,Wagers AJ,et al.Rejuvenation of aged progenitor cells by exposure to a young systemic environment[J].Nature,2005,433(7027):760-764.
  • 10Sethe S, Scutt A, Stolzing A. Aging of mesenchymal stemcells[ J]. Ageing Res Rev, 2006 , 5(1):91-116.

共引文献9

同被引文献21

  • 1卢俊杰,马陈,朱伟,杨艳萍,徐晓娟,沈霖,帅波.加味青娥丸对糖皮质激素诱导骨质疏松小鼠骨组织外泌体miRNA表达谱的影响[J].中华骨质疏松和骨矿盐疾病杂志,2022,15(6):651-660. 被引量：3
  • 2Li J, Dong J, Zhang ZH, et al. miR-10a restores human mesenchymal stem cell differentiation by repressing KLFg [ J ]. J Cell Physiol, 2013, 228 (12) : 2324-2336.
  • 3Jang JS, Jeon HS, Sun Z, et al. Increased miR-708 ex- pression in NSCLC and its association with poor survival in lung adenocarcinoma from never smokers[ J]. Clin Cancer Res, 2012, 18(13) :3658-3667.
  • 4Kang SK, Shin IS, Ko MS, et al. Journey of mesenchymal stem cells for homing: strategies to enhance efficacy and safety of stem cell therapy [ J ]. Stem Cells Int, 2012,2012.342968.
  • 5Collins-Hooper H, Woolley TE, Dyson L, et al. Age-re- lated changes in speed and mechanism of adult skeletal muscle stem cell migration [ J ]. Stem Cells, 2012, 30 (6) :1182-1195.
  • 6Bustos ML, Huleihel L, Kapetanaki MG, et al. Aging mesenchymal stem cells fail to protect because of impaired migration and antiinflammatory response [ J ]. Am J Respir Crit Care Med, 2014, 189(7) :787-798.
  • 7Saini S, Yamamura S, Majid S, et al. MicroRNA-708 in- duces apoptosis and suppresses tumorigenicity in renal cancer cells[ J]. Cancer Res, 2011,71 (19) :6208-6219.
  • 8Ryu S, Mcdonnell K, Choi H, et al. Suppression of miR- NA-708 by potycomb group promotes metastases by calci- um-induced cell migration [ J ]. Cancer Cell, 2013, 23 ( 1 ) :63-76.
  • 9Lee H J, Finkelstein D, Li X, et al. Identification of transmembrane protein 88 (TMEM88). as a dishevelled- binding protein [ J ]. J Biol Chem, 2010, 285 ( 53 ) : 41549-41556.
  • 10Palpant N J, Pabon L, Rabinowitz JS, et al. Transmem- brane protein 88: a Wnt regulatory protein that specifies cardiornyoeyte development [ J ]. Development, 2013, 140 (18) :3799-3808.

引证文献3

二级引证文献4

中国病理生理杂志
2014年 第12期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部