UTX在肾癌中的高表达及其临床意义

Clinical significance of high expression with UTX in renal cell carcinoma

目的:检测UTX(ubiquitously transcribed TPR gene on the X chromosome)在肾透明细胞癌中的表达及其临床意义。方法:收集临床诊断明确肾透明细胞癌患者组织标本45例,提取组织mRNA,制作组织切片,采用实时定量PCR和免疫组织化学分析UTX在肾癌组织和癌旁组织表达的差异,并分析其表达改变与临床病理参数之间关系。结果:实时定量PCR结果显示,UTX mRNA在肾癌组织中的表达明显高于癌旁组织[(0.883 2±0.703 8 vs.0.199 7±0.140 0,P<0.05],免疫组织化学结果同样表明肾癌组织UTX表达水平明显高于癌旁组织(P<0.05),UTX在肾癌组织中的蛋白评分高出癌旁组织4倍[12±4 vs.3±3,P<0.05]。UTX在肾癌组织中的表达与性别、年龄、肿瘤大小、TNM分期无明显关系,但与病理分级关系密切(P=0.004)。结论:UTX在肾透明细胞癌中表达增高,并且与肾癌分级相关。

Objective：To investigate the expression and significance of ubiquitously transcribed TPR gene on the X chromosome （UTX） in renal cell carcinoma （RCC） tissues,then to explore the relationship between UTX expression and renal cancer pathologic characteristics.Methods：In the study,45 patients with dignosed renal cell carcinoma clinical samples were collected in Peking University Shenzhen Hospital.Total RNA and protein were extracted from the cancer tissues and adjacent normal tissues.UTX expression of cancer tissues and adjacent normal tissues was detected on both mRNA and protein levels using real time-PCR and IHC,respectively.And the relationship between UTX expression and the 45 patients＇ clinical characteristics was analyzed.Results：The mRNA level of UTX in cancer tissues （C） was 4.4 folds,higher than that of the adjacent normal tissues（N） [0.883 2 ±0.703 8 vs.0.199 7 ± 0.140 0,P ＜ 0.05].The protein expression of UTX in cancer tissues was up-regulated,and the protein score of cancer tissues was 4 folds,change compared with adjacent normal tissues[12 ±4 vs.3 ±3,P ＜ 0.05].The expression of UTX was associated with pathological grade （P =0.004） but without gender,age,tumor size and TNM stage.Conclusion：UTX is up-regulated in RCC tissues and the expression of UTX is associated with pathological grade,illustrating that UTX may play an important role in renal cancer progression.