p53转染黑色素瘤细胞修饰的树突状细胞疫苗体外抗肿瘤作用的研究

Study of Anti-tumor Immunity In Vitro Induced by DC Vaccines Loaded by p53-modified Tumor Antigens

利用野生型p53质粒转染黑色素瘤B16细胞,反复冻融法提取p53修饰的肿瘤抗原(p53-Ag),将抗原体外冲击同基因小鼠骨髓来源的树突状细胞(dendritic cells,DC)制备特异性DC肿瘤疫苗;观察DC诱导的淋巴细胞增殖反应和细胞毒性T淋巴细胞(cytotoxic T lymphocytes,CTL)对黑色素瘤细胞的细胞毒效应,分析其诱导肿瘤抗原特异性免疫应答的机制。结果显示,p53-肿瘤抗原冲击的DC可显著刺激淋巴细胞增殖,其诱导的CTL效应对肿瘤细胞也有很好的杀伤效果。

Tumor antigen （p53-Ag） was extracted from mouse B16 melanoma cells transfected with the wild-p53 plasmid by freeze-thaw method. Then it was loaded onto dendritic cells （DC） harvested from the bone marrow to obtain DC tumor vaccine. The antigen-specific killing effect of cytotoxic T lymphocytes （CTL） activated by DC vaccine on cancer cells in vitro and the ability to induce proliferation of T cells were detected by MTT assay. The experiment results showed that p53-modified DC vaccine significantly up-regulated the proliferation of T lym- phocytes and the killing rate by CTL on cancer cells.