芍药苷对人黑素细胞生物活性及迁移的影响

Effects of paeoniflorin on biological activity and migration of human melanocytes

目的:确定芍药苷对人黑素细胞生物活性及迁移的影响。方法:体外培养人表皮黑素细胞,分别使用MTT法、L-DOPA法、NaOH法、Transwell小室法检测不同浓度的芍药苷对黑素细胞的增殖活性、酪氨酸酶活性、黑素合成以及黑素细胞迁移的影响。结果:芍药苷能显著促进黑素细胞增殖和上调酪氨酸酶活性,且10μ/mL是产生这两方面作用的最佳浓度;芍药苷也能促进黑素的合成,50μg/mL时作用最显著。芍药苷在20μg/mL时显著促进黑素细胞迁移。各组与空白对照组比较差异均有显著性(P<0.05)。结论:芍药苷单体可能是白芍中促进黑素细胞迁移的主要活性成分。

Objective： To determine the effects of paeoniflorin on biological activities and migration of mel- anocytes. Methods： A melanocytes culture system was established and proliferation activity, tyrosinase activi- ty, melanogenesis and migration were detected by MTY, L-DOPA, NaOH method and transwell assay respec- tively. Results： The paeoniflorin improved the melanocytes proliferation and tyrosinase activity, in which the effects were the most obvious at the concentration of 10 μg/mL. The paeoniflorin promoted the synthesis of melanin, which was the most obvious at the concentration of 50 μg/mL. Paeoniflorin promoted the migration of melanocytes obviously at the concentration of 20 μg/mL. There were significant differences between the paeon- iflorin groups and the control groups （P〈0.05）. Conclusion： The paeoniflorin is the principal active compo- nent of radix paeoniae alba that promoting the migration of melanocytes.