山东产区黄芩HPLC定量指纹图谱研究

Study On Quantitative HPLC Fingerprint of Scutellaria baicalensis Georgi

目的:建立山东产区黄芩药材定量指纹图谱,为黄芩药材的真伪鉴别及质量控制提供方法支持。方法:采用Agilent ZORBAX SB-C18色谱柱,乙腈-水(含1%甲酸、20 mmol·L-1甲酸铵)为流动相进行梯度洗脱,流速为0.8 mL·min-1,柱温为25℃,检测波长为274 nm,进样量5μL,进行了10批次黄芩药材的分析。结果:该分析方法具有良好的精密度、重现性,10批黄芩指纹图谱有17个共有峰,4个特征峰,采用ESI-TOF/MS对4个特征峰进行了指认,10个批次山东产黄芩样品中6种黄酮类化合物含量分布规律相似,其相似度系数均大于0.955 7。结论:黄芩HPLC定量指纹图谱有望成为黄芩样品真伪鉴别及质量控制的有力工具。

Objective ： A quantitative method had been used for the quality high performance liquid chromatography （ HPLC ） fingerprinting evaluation and discrimination of Scutellaria baicalertsis Georgi. Methods： The chromatographic fingerprints were determined by injecting 5 μL of the sample solution each time on C is column（ Agilent ZORBAX SB-C18 column）with the gradient elution solvent system composed of 20 mmol. L- 1 HCOONH4 ＋ 1% HCOOH and acetonitrile. The flow rate was 0. 8 mL. min -1, the colunm temperature was maintained at 25 ℃ and the signals were acquired at 274 nm. Results： It was indicated that the developed HPLC method was simple, accurate and reliable for the development of S. baicalensis fingerprint. The HPLC fingerprinting for ten batches samples tested contained the same seventeen peaks, and it could be used to discriminate different origins of S. baicalensis samples combined with similarity analysis. Conclusion： The HPLC fingerprinting technique is an important tool in identification and quality evaluation of S.baicalensis.