红芪与黄芪中两个异黄酮类成分含量的比较研究

Comparative Study of Formononetin and Calycosin Content in Hedysari Radix and Astragali Radix

目的：通过高效液相色谱法,对红芪和黄芪药材进行鉴别和定量分析,为药材质量评价提供科学依据.方法：色谱柱为TC-C18（2）柱（250 mm ×4.6 mm,5 μm）,TC-C18保护柱（10 mm× 4.6 mm,5μm）,流动相为乙腈-0.01％磷酸水溶液（梯度洗脱）,流速为1.0 mL·min-1,检测波长为248 nm,柱温为30℃,进样量为10 μL.结果：15批红芪样品中芒柄花素的质量分数均高于毛蕊异黄酮,其中芒柄花素质量分数在77.51 ～ 424.94 μg·g-1,毛蕊异黄酮质量分数在2.27 ～26.13 μg·g-1;而7批黄芪中芒柄花素的质量分数均低于毛蕊异黄酮,其中芒柄花素质量分数在4.84 ～29.94 μg·g-1,毛蕊异黄酮质量分数在12.95 ～62.98 μg·g-1.结论：毛蕊异黄酮和芒柄花素的相对质量分数可作为红芪与黄芪的定性鉴别和质量评价的主要特征之一.

Objective： To do identification and qualitative analysis on Hedysari Radix andAstragali Radix by HPLC, and provide a scientific basis for the quality evaluation. Methods： The sample was separated on TC-C18 （2）（250 mm ×4. 6 mm, 5 μm）column. The mobile phase consisted of aeetonitrileand 0. 01% phosphoric acid solution （gradient elution）at a flow rate of 1.0 mL. min-1. The UV detection wave length was set at 248 nm. The column temperature was set at 30 ℃. The injection volume was 10μL. Results： Formononetin content was higher than calycosin in Hedysari Radix. Formononetin content was between 77.51 - 424. 94 μg . g-1, and calycosin between 2. 27 - 26. 13μg . g-1 , but the contents inAstragali Radix were exactly opposite. Formononetin content was between 4. 84 -29. 94 μg. g-1, and calycosin between 12. 95 - 62. 98 μg.g-1. Conclusion： The relative content of formononetin and calycosincan be main features of qualitative identification and quality evaluation on Hedysari Radix and Astragali Radix.