穿山龙提取物的纯化工艺研究

Study on Purification Process of Extracts from Dioscorea nipponica

目的:研究并建立穿山龙提取物的纯化工艺。方法:以总皂苷和薯蓣皂苷综合评价为指标,利用大孔吸附树脂纯化穿山龙提取物。结果:以生药质量浓度为1 g·mL-1的上柱液,通过D-101型大孔吸附树脂,依次用4 BV水,体积流量为1 BV·h-1,5 BV 65%乙醇,体积流量为2 BV·h-1洗脱,并收集65%乙醇洗脱流分,减压干燥,分离转移率平均分别为84.8%、84.2%。结论:该方法稳定可行,能较好地纯化穿山龙提取物。

Objective： This study aimed to establish the best purification technique for the extraction process of Dioscorea nipponica. Methods： Use the total saponins and diosgenin comprehensive evalution as index, and to purify the extraction from D. nipponica by means of macroporous adsorption resin. Results： The results showed that after going through the D101 macroporous adsorption resin, eluting by means of 4BV water（1 BV.h-1） , 5BV 65% ethanol（2 BV.h-1）, and collecting the dried 65% elute ethanol, the average separation of transfer rate is 84. 8% and 84. 2%. Conclusion： The process is proved reliable and stable to purify the astraction from D. nipponica.