自发性高血压大鼠肠系膜微静脉白细胞-内皮细胞相互作用和微淋巴管收缩特性的研究

Venular Leukocyte Rheology and Microlymphatic Vessel Contractility in Spontaneously Hypertensive Rats Mesentery

目的：探讨自发性高血压大鼠（Spontaneously Hypertensive Rat,SHR）肠系膜微静脉白细胞-内皮细胞相互作用和微淋巴管收缩的特性。方法：取8周龄Wistar大鼠、8周龄SHR（SHR8W）和13周龄SHR（SHR13W）,麻醉、固定并暴露肠系膜后,微循环显微镜下观察肠系膜微循环并录像;回放录像,计算微静脉白细胞滚动数和滚动的白细胞-内皮细胞接触时间（Rolling leukocyte-endothelial contact time,RLECT）,用Vas Track自动测量系统对微淋巴管口径进行动态测量,并计算微淋巴管收缩特性指标。结果：SHR13W的白细胞滚动数显著低于Wistar;SHR8W和SHR13W的RLECT均显著低于Wistar,且SHR13W的RLECT显著低于SHR8W;进一步按照管径分级后,三组间白细胞滚动数在10-20μm管径级别下未见差异;各个管径级别下,SHR8W和SHR13W的RLECT均未见差异。SHR13W的淋巴管收缩分数显著低于Wistar和SHR8W;SHR8W及SHR13W的总收缩活性指数均显著低于Wistar;SHR13W的淋巴管动力指数显著低于Wistar。结论：SHR肠系膜微静脉白细胞滚动数及RLECT减少,其中白细胞滚动数在不同管径级别微静脉中的分布不均匀,而RLECT随SHR周龄降低,意味着SHR淋巴管收缩功能降低。

Objective： To explore the venular leukocyte-endothelium interaction （LEI） and micro lymphatic vessel contractility in SHR mesentery. Methods： Animals included 8-week-old Wistar rats, 8-week-old SHRs and 13-week-old SHRs. All rats were male. Following anesthesia, all these rats were fixed before the exteriorization of the mesenteric tissue. And then, microscope was used for recording a video of mesenteric microcirculation. According to the video, rolling leukocyte number and RLECT were calculated. Diameter and contract frequency of micro lymphatic vessel were measured automatically by VasTrack software for calculating the lymphatic contractility indices. Results： The number of rolling leukocyte in SHR13W is significantly lower than that of Wistar before diameter gradation was conducted. The difference among the three groups was not found in the 10 -20 vm range after diameter gradation. The statistical difference in RLECT between Wistar and either SHR8W or SHR13 was found again after diameter gradation, but the significant difference between SHR8W and SHR13W found before diameter gradation was not found after diameter gradation. Lymphatic contraction fraction in SHR13W was significantly lower than that in Wistar and SHR8W. Both SHR8W and SHR13W had a decreased total contraction index compared with that of Wistar. Lymphatic dynamic index in SHR 13W was significantly lower than that of Wistar. Concltmion： Venules in SHR mesentery have a decreased number of rolling leukocyte and RLECT, with its number of rolling leukocyte distributed not evenly among venutes of different diameter ranges, while RLECT decreases with age in SHR, which means that Lymphatic vessel contract ability of SHR is decreased.