NUCB2/nesfatin-1对小鼠摄食行为的调控及机制

Regulatory Effect of NUCB2/nesfatin-1 on Feeding Behavior in Mice and the Potential Mechanism

目的：探讨NUCB2/nesfatin-1对小鼠摄食行为的调控及机制。方法：利用侧脑室埋管,免疫组化染色等方法,探讨侧脑室和外周注射nesfatin-1对小鼠摄食行为的影响。结果：侧脑室注射不同剂量nesfatin-1（0.3μg,1μg,3μg）,注药后4 h夜间进食量明显减少,且呈显著剂量依赖关系（t=2.61～4.78,P〈0.05～0.01）,侧脑室注射3μg nesfatin-1,小鼠前3小时累积摄食量明显降低（t=8.69～10.73,P〈0.01）,且持续降低12小时（t=2.64,P〈0.05）,同时餐间间隔时间明显延长（t=2.66,P〈0.05）,每分钟/1-4 h进食量明显降低（t=2.63,P〈0.05）,且进食每克食物所用时间明显增加（t=3.02,P〈0.05）。在下丘脑弓状核,外侧区和背内侧核均有NUCB2/nesfatin-1免疫阳性神经元表达。皮下或腹腔注射nesfatin-1,小鼠进食量和进食行为均无显著改变（P〉0.05）。结论：中枢nesfatin-1可抑制小鼠摄食行为。

Objective： To investigate the regulatory effect of NUCB2/nesfatin-1 on feeding behavior in mice and the potential mechanism. Methods： Nesfatin-1 was injected intracerebroventricularly （icv） by lateral ventricle buffed pipe and peripherally （intraperitoneal, i.p, subcutaneous, s.c） on mice. Nesfatin-1 immunostaining was performed. Results： Nesfatin-1 （0.3, 1 or 3μg/mouse, icv） caused a dose-dependent reduction of 4-h dark phase food intake （t-=-2.61--4.78, P〈0.05-0.01）. Nesfatin-1 （3μg/mouse, icy） led to the reduction of 3-h cumulative food intake （t=-8.69-10.73,P〈0.01） lasting for 12 h （t=-2.64, P〈0.05）, significantly increased inter-meal intervals （t=-2.66, P〈0.05） and time spent in meals （t=-3.02, P〈0.05） and significantly decreased meal size （t=-2.63, P〈0.05）. NUCB2/nesfatin-l-immunopositive neurons were found in the hypothalamic arcuate nucleus, lateral and dorsomedial hypothalamic nuclei. When injected peripherally, neither food intake nor any feeding mierostructure parameters were altered. Conclusion： The central nesfatin-1 leads to a dose-dependent reduction of food intake in mice.