p16和IMP3在宫颈上皮病理改变中的表达及意义

Expression and significance of p16 and IMP3 in cervical intraepithelial neoplasia and cervical squamous cell carcinoma

目的研究p16和IMP3在宫颈上皮内瘤变(CIN)和鳞状细胞癌中的表达,探讨它们在CIN和鳞状细胞癌中的诊断价值。方法采用免疫组织化学方法检测20例慢性宫颈炎、97例CINⅠ、78例CINⅡ、68例CINⅢ和24例宫颈鳞状细胞癌中p16和IMP3的表达。结果 p16在慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ和宫颈鳞状细胞癌阳性表达率分别为10%、55.7%、97.4%、97.1%和91.7%。p16在慢性宫颈炎与低级别CIN中的阳性表达率有显著性差异(P<0.01),在高级别CIN和宫颈鳞状细胞癌中的表达明显高于低级别CIN和慢性宫颈炎,有显著性差异(P<0.01);而在高级别CIN和宫颈鳞状细胞癌中的阳性表达率没有差异(P>0.05)。IMP3在慢性宫颈炎和CINⅠ中不表达,在宫颈高级别上皮内瘤变(CINⅡ和CINⅢ)的阳性表达率分别为16.7%和23.5%,在宫颈鳞状细胞癌中的阳性表达率为91.7%,有显著性差异(P<0.01)。结论 p16的表达强度在宫颈上皮内瘤变到宫颈鳞状细胞癌的发展过程中逐步升高,IMP3表达主要在病变的晚期阶段。p16和IMP3结合应用有助于宫颈上皮内瘤变和宫颈鳞状细胞癌的诊断和分级。

Objective To study the expression of p16 and IMP3 in cervical imtraepithelial neoplasia and cervical squamous carcinoma,and to explore the diagnosis value of them. Methods The expression of p16 and IMP3 were detected by immunohisto-chemical staining in 20 cases of chronic cervicitis,97 cases of CINⅠ,78 cases of CINⅡ,68 cases of CINⅢand 24 cases of cer-vical squamous cell carcinoma. Results The positive rate of p16 expression in chronic cervicitis,CINⅠ,CINⅡ,CINⅢand cervi-cal squamous cell carcinoma was 10%,55.7%,97.4%,97.1% and 91.7%,respectively. The positive rate of p16 expression in chronic cervicitis and low-grade CIN was significant difference（P〈0.01）. The posive expression rate of p16 in high-grade CIN and cervical squamous cell carcinoma were significantly higher than chronic cervicitis and low-grade CIN ,the difference were signifi-cant （P〈0.01）. The posive expression rate of p16 in high-grade CIN and cervical squamous cell carcinoma compared with no cignificant difference （P〉0.05）. The negative of IMP3 expression in chronic cervicitis and low-grade CIN. The positive rate of IMP3 expression in CINⅡ,CINⅢ and cervical squamous cell carcinoma was 16.7%,23.5% and 91.7%,respectively. There were significant differences （P〉0.01）. Conclusion The expression levels of p16 were increased with cervical epithlelial malignant transformation progress and the expression of IMP3 was the lately event of development of cervical squamous carcinoma. Using p16 and IMP3 may be helpful for the diagnosis and of grade of CIN and cervical squamous cell carcinoma.