摘要
为筛选出适合鸡爪苓菌丝体中总RNA提取的方法,采用Trizol法、Trizol改良1、2法、CTAB改良法提取鸡爪苓菌丝体的总RNA。结果表明：Trizol改良1、2法提取总RNA的质量与纯度优于Trizol法和CTAB改良法,LD-PCR成功合成鸡爪苓菌丝体cDNA产物,分布在500~5 000bp,满足cDNA文库的建库要求。
In order to select suitable method for extracting total RNA from mycelium of chicken-claw- shaped Polyporus umbellate, Trizol method, improved Trizol method 1 and 2 and CTAB method were compared. The results showed that the quality and purity of total RNA extracted using improved Trizol method 1 and 2 were better than those using Trizol method and the improved CTAB method. Using the total RNA from chicken-claw-shaped Polyporus umbellate mycelium, cDNAs were successfully synthe- sized by LD-PCR. Lengths of the cDNAs varied from 500 to 5 000 bp,which met the requirements of eD- NA library construction.
出处
《延边大学农学学报》
2014年第4期297-301,共5页
Agricultural Science Journal of Yanbian University
基金
国家自然科学基金资助项目(31160014)
关键词
鸡爪苓
菌丝体
总RNA
提取方法
chicken-claw-shaped Polyporus umbellate
mycelium
total RNA
extraction method
