饥饿状态下CVB3对Hela细胞4EBP1表达的影响

4EBP1 expression in He La cells infected with CVB3 under starvation

目的观察饥饿状态下柯萨奇病毒B3(CVB3)感染Hela细胞后对4EBP1表达的影响。方法用RT-PCR法检测CVB3感染后非饥饿及饥饿状态下Hela细胞中4EBP1的表达。结果饥饿与非饥饿状态下对照组及CVB3感染组(病毒组)Hela细胞中均有4EBP1表达,病毒组4EBP1表达降低(P<0.05),随病毒感染时间延长,4EBP1表达下降更明显(P<0.05);非饥饿组4EBP1表达低于饥饿组(P<0.05)。结论 CVB3感染Hela细胞后4EBP1表达下降,随时间的延长下降更明显,饥饿促进4EBP1的表达。

[Objective] To observe the expression of 4EBP1 in HeLa cells infected with CVB3 under starvation. [Methods] The expressions of 4EBPI in Hela cells infected with CVB3 with or without nutrition were determined by reverse transcription PCR. [Results] The expressions of 4EBP1 were found in both groups of the control and virus infected under starvation. Meanwhile, the 4EBP1 expressions in the virus infected groups were lower than that in the controls （P 〈0.05）, and which in viral infected groups were decreased as the infected time increasing. Com- pared with the starved groups, the 4EBP1 expressions decreased more significantly in the controls （P 〈0.05）. [Con- clusions] The expressions of 4EBP1 in Hela cells, which were decreased after CVB3 infected and further deceased with the infected time increasing, were promoted by the serum starvation.