烟气脱硝尾液厌氧氨氧化处理中微生物群落结构分析

Microbial community in the Anammox process of thermal denitration tail liquid

采用厌氧序批式反应器处理火电厂烟气脱硝尾液并研究厌氧氨氧化微生物群落结构。通过扫描电镜观察发现,优势菌群为椭圆形的球菌且具有下凹的火山口状,直径约为0.7μm。通过污泥总DNA提取、PCR扩增、阳性克隆验证和测序等分子生物学手段得到反应器内菌群16S r RNA基因序列,建立系统发育树和克隆文库。结果表明,在细菌通用引物27F-1 492R PCR扩增的体系中,得到85个克隆子,分为21个OTU,各菌群所占的比例为:变形菌门Proteobacteria:61.18%;酸杆菌门Acidobacteria:17.65%;绿菌门Chlorobi:8.24%;绿弯菌门Chlorofexi:5.88%;芽单胞菌门Gemmatimonadetes:3.53%;硝化螺旋菌门Nitrospirae:2.35%;浮酶状菌门Planctomycetes:1.18%。而在厌氧氨氧化菌特异引物pla46rc-630r;AMX368-AMX820 PCR扩增的体系中有45个克隆子,分为3个OTU,其中Candidatus brocadia sp.占有95.6%,未知菌种4.4%。

An anaerobic sequencing batch reactor（ASBR） was used to treat thermal denitration tail liquid and microbial community was studied. Activated sludge was taken from the reactor for scanning electron microscope analysis. The images showed that the dominant cells in the flora were oval cocci. Its diameter was about 0.7 μm. Through a series of molecular biology methods such as extracting total DNA from the sludge, PCR amplification, positive clone authentication and sequencing, we obtained the 16 S r DNA sequences of the flora. Phylogenetic tree and clone library were established. The universal bacteria primers of 27F-1492 R PCR amplification system obtained 85 clones and could be divided into 21 OTUS. The proportions were as follows： Proteobacteria 61.18%; Acidobacteria 17.65%; Chlorobi 8.24%; Chlorofexi 5.88%; Gemmatimonadetes 3.53%; Nitrospirae 2.35% and Planctomycetes 1.18%. The specific anammox bacterial primers of pla46rc-630 r and AMX368-AMX820 PCR amplification system obtained 45 clones. They were divided into 3 OTUS. Candidatus brocadia sp. occupied 95.6% and unknown strains occupied 4.4%.