贵州地区深部感染来源白念珠菌的微卫星多态性

Microsatellite polymorphism of Candida albicans isolated from invasive infection sources in Guizhou region,China

目的调查贵州地区各类深部感染来源的白念珠菌CAI区微卫星基因型分布特点。方法收集95株贵州地区深部感染来源的白念珠菌,采用CAI区的单链构型多态性(SSCP)图谱结合GeneScan分析进行CAI区微卫星分型;计算多态性信息含量(PIC)和Nei氏遗传距离,以类平均法(UPGMA)进行聚类分析。结果 95株贵州地区深部感染来源的白念珠菌被鉴定为40种CAI区微卫星基因型,其中有7种属至今尚未报道过的CAI区微卫星基因型;PIC为0.9046;根据白念珠菌CAI区微卫星基因型间的Nei氏遗传距离将菌株分为7大类群,其中慢性阻塞性肺疾病和获得性免疫缺陷综合征(AIDS)来源白念珠菌部分菌株在某一类群呈集中分布,其余部分菌株在各类群中呈散在分布,而肿瘤来源菌株则在聚类分析中呈相对集中分布的特点。结论贵州地区深部感染不同来源的白念珠菌菌株具有丰富的遗传多样性;不同来源的白念珠菌的CAI区微卫星基因型在微生态进化上具有相关性。

Objective To survey the microsatellite polymorphism of Candida albicans（C.albicans）strains isolated from invasive infection sources in Guizhou region.Methods Ninety-five independent C.albicans strains isolated from invasive infection sources in Guizhou region were investigated based on single-strand conformation polymorphisms （SSCP）and GeneScan analysis.Genotypes were identified by microsatellite locus CAI polymorphism analysis.The polymorphic information content （PIC）and Neis genetic distance were computed by software PowerMarker V3 .25 .In addition,phylogenetic tree was constructed based on UPGMA methods.Results Among the 95 C.albicans strains isolated from invasive infection sources,40 distinct CAI genotypes with various patterns were identified by GeneScan analysis,and there were 7 novel CAI microsatellite genotypes which were identified in Guizhou province.The PIC was 0.904 6.Seven clusters were divided in the UPGMA phylogenetic tree with Neis the genetic distance,in which the strains from chronic obstructive pulmonary disease and AI DS were scattered in different clusters,while strains from tumor source were gathered in some relatively close clusters.Conclusion The C.albicans strains isolated from various invasive disease sources shows abundant genetic diversity characteristics.The CAI microsatellite genotypes of C.albicans from different sources are correlated to their micro-ecological evolution evidence.