摘要
采用电解法制备新型、高效纳米银膜作为近红外表面增强拉曼散射(NIR-SERS)基底,检测了22例健康女性和22例宫颈癌患者氧合血红蛋白的NIR-SERS光谱。对比宫颈癌患者与健康女性氧合血红蛋白NIR-SERS光谱,发现两者区别明显。利用多变量统计分析方法得到宫颈癌患者与健康女性氧合血红蛋白的NIR-SERS光谱在472、662、720和1209 cm-1谱峰处区别最为明显,诊断的特异性与灵敏度均为86.4%。由谱峰归属分析得知,宫颈癌患者血液中高铁血红蛋白吡咯环折叠振动、氧合血红蛋白吡咯环的对称变形振动、反对称变形振动和吡咯环间CmH基团的变形振动等振动模式与健康女性相比有明显的减少。研究表明,NIR-SERS技术结合多变量统计分析方法可以较好地区分宫颈癌患者和健康女性氧合血红蛋白,有望发展成为一种新型的宫颈癌临床诊断工具。
A new type of Ag nanofilm with high efficiency is prepared and used here, acting as a near-infrared surface-enhanced Raman spectroscopy (NIR-SERS) substrate to detect oxyhemoglobin (OxyHb) for 22 cervical cancer patients and 22 healthy females. NIR-SERS results show that there is striking spectral difference of the OxyHb between healthy females and cervical cancer patients. Meanwhile, principal component analysis (PCA) combined with independent sample T-test analysis is employed to analyze the measured NIR-SERS spectra, and it is found that the measured NIR-SERS spectra of the two groups are separated into two distinct clusters in terms of the sensitivity of 86.4% and the specificity of 86.4%. Tentative assignments of the Raman bands of the measured NIR-SERS spectra are also performed, and the results suggest that cancer specific changes at molecular level, including pyrrole ring and vibration mode of the OxyHb molecules are significant. The NIR-SERS detection of OxyHb for cervical cancer patients based on PCA combined with independent sample T-test is expected to develop into a new diagnostic tool for cervical cancer.
出处
《中国激光》
EI
CAS
CSCD
北大核心
2015年第1期332-341,共10页
Chinese Journal of Lasers
基金
国家自然科学基金(11364001
10864001
11064001)
云南省应用基础研究计划青年项目(2013FD044)
楚雄师范学院学术后备人才资助项目(13XJRC19)
关键词
光谱学
近红外表面增强拉曼散射
主成分分析
宫颈癌
氧合血红蛋白
spectroscopy
near-infrared surface-enhanced Raman spectroscopy
principal component analysis
cervical cancer
oxyhemoglobin