糖尿病性痛大鼠前列腺酸性磷酸酶的时空表达

Spatio-temporal expression of prostatic acid phosphatase in rats with diabetic mechanical allodynia

目的：探讨糖尿病性痛（Diabetic neuropathic pain,DNP）模型大鼠中前列腺酸性磷酸酶（Prostatic acid phosphatase,PAP）的时空表达特点。方法：采用链脲佐菌素（Streptozocin,STZ）腹腔注射诱导建立DNP大鼠模型,应用Von-Frey细丝法和Hargreaves热辐射法观察大鼠的机械痛和热痛变化。免疫组化法检测腰膨大节段（L4-5）脊髓背角（Spinal dorsal horn,SDH）和背根神经节（Dorsal root ganglion,DRG）中PAP随DNP病程发展的表达。结果：DNP大鼠的机械痛阈和热痛阈在造模后第7天开始明显降低,并维持至第35天,与对照组相比有显著性差异（P〈0.05）;造模7d后模型组大鼠DRG神经元中PAP表达明显减少,14d和21d时表达进一步降低,并持续至35d;而模型组大鼠SDH中PAP的表达从造模后14d开始逐渐减少至35d,但PAP表达在SDH中的变化较DRG晚1周。结论：随着DNP大鼠病程进展,PAP在DRG和SDH中的表达均呈现减少的趋势,且与痛敏变化几乎平行;提示PAP可能是DNP中一种具有调控镇痛效应的分子。

Objective：To investigate the spatio-temporal expression of prostatic acid phosphatase（PAP）in rats with diabetic neuropathic pain（DNP）.Methods：DNP rat model was induced by intraperitoneal（ip）injection of streptozocin（STZ）.The Von-Frey filaments and Hargreaves tests were applied to identify the changes of the paw withdrawal threshold（PWT）and paw withdrawal latency（PWL）in DNP rats.Immunohistochemistry was employed to detect the spatio-temporal expression of PAP in the spinal dorsal horn（SDH）and dorsal root ganglion（DRG）on different time points after i.p.injection of STZ,respectively.Results：Compared with control group,i.p.injection of STZ induced significant mechanical allodynia and thermal hyperalgesia indicated by the reduced PWT and PWL from 7d,and maintained to 35d（P〈 0.05）.The expression of PAP in DRG neurons was significantly decreased from 7dto 21d（P〈 0.05）,then slightly increased from 28 dto 35d;while that in SDH was markedly decreased from 14 dto 35d（P〈0.05）,compared with control group,but the changes in SDH were 1wlater than that in DRG..Conclusion：With the progress of DNP,the expression of PAP was significantly decreased in the DRG and SDH,which was almost parallel with the pain process.These results have suggested that PAP may play a key role in the alleviation pain of the DNP.