高效液相色谱法测定花生内源激素的改进研究

Study on the Improvement of HPLC Method for Analyzing Endogenous Hormone in Peanut

本实验以花生籽仁为材料,对提取、纯化和利用高效液相色谱法分析测定花生籽仁中内源激素生长素(IAA)、脱落酸(ABA)、赤霉素(GA)和细胞分裂素(6-BA)含量的方法进行改进,针对花生籽仁中脂肪、酚类含量高等特点,对以往的HPLC分析植物内源激素的植物材料前处理方法进行了改进。试材用液氮研磨后以80%预冷的甲醇浸提,提取液利用旋转蒸发仪浓缩,氯仿去除色素,乙酸乙酯提取激素,交联聚乙烯吡咯烷酮(PVPP)去除酚类杂质,以甲醇和水为流动相,254nm吸收波长下检测内源激素含量。

This experiment improved the method of extraction, purification and analysis with high-performance liquid chromatography （ HPLC） on endogenous hormones auxin （IAA）, abscisic acid （ABA）, gibberellic acid （GA） and 6-Benzylaminopurine （6-BA） content in peanut kernel.To deal with the existence of large amount of fat and phenolic, the test materials were grinded in liquid nitrogen and lixiviated in 80% methanol, then pigment was eliminated in chloroform, phytohormones were extracted in ethyl acetate, phenolic was compounded in polyving pyrrolidone （PVPP）.Detection was made with UV detector at 254 nm.