摘要
RNA干扰(RNA interference,RNAi)是一种由与靶基因同源的双链RNA引起的,具有序列特异性的转录后基因沉默技术。目前,RNAi已被广泛应用于基因功能的研究中。为了使基因沉默具有时空特异性,可以通过构建基于Cre重组酶的Cre/lox P系统、可被小分子药物诱导的Tet诱导系统以及两者组合形成的高级诱导RNAi系统来条件性的控制小发夹状RNA(sh RNA)的表达,使其靶基因的表达可受外界调控,在特定组织细胞中或者特定的生长发育时期被抑制,从而更好地进行基因功能研究。目前,条件性RNAi基因沉默策略具有多样化,精确性和组合式的特点。本文就条件性RNAi基因沉默技术的多元策略做一综述。
RNA interference （RNAi） is a technology of sequence-specific post＇transcriptional gene silencing, which has been extensively applied for deciphering gene function. In order to achieve temporal and spatial specificity of gene silencing, conditional short hairpin RNA （shRNA） expression vectors can be generated based on DNA recombinase, e.g. the Cre/loxP system, and small molecule drug-inducible gene expression like the Tet inducible system, allowing for gene silencing in certain type of cells or during certain phase of the cells or tissues. This paper reviewed the conditional gene silencing systems currently available or in development.
出处
《现代生物医学进展》
CAS
2015年第3期547-550,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81272646)
