紫杉醇-顺铂联合药物控释系统对肺腺癌细胞系A549细胞生长的抑制作用

Antitumor activity of paclitaxel or / and cisplatin drug delivery system against lung cancer cells A549 in vitro

目的观察以聚碳酸亚丙酯乳液作为纺丝液,采用静电纺丝技术,负载紫杉醇和顺铂制备的载药纤维控释系统对体外培养的肺腺癌细胞系A549的抑制率,为进一步的动物实验奠定基础,并探讨用于肺癌治疗的可行性。方法体外培养肺腺癌细胞系A549。分别比较紫杉醇、顺铂和两药联合纤维载药控释系统与其相应的裸药对照组对A549的抑制率。肿瘤细胞生长抑制率测定采用酶标仪测定吸光度(490 nm波长)。药物对肿瘤细胞抑制率=(对照组吸光度-实验组吸光度值)/对照组吸光度。结果紫杉醇单药、顺铂单药和两药联合的载药纤维控释系统对肺癌细胞A549的生长的抑制率均强于相应的裸药组。顺铂单药和两药联合的载药纤维控释系统的肿瘤细胞抑制率随药物浓度的增加而呈明显的上升趋势。结论聚碳酸亚丙酯负载紫杉醇和/或顺铂静电纺丝制备控释给药系统可显著抑制体外培养的肺腺癌细胞系A549的生长。该给药系统有望实现抗癌药物解剖靶向控释给药。

Objective To observe paclitaxel and/ or cisplatin loaded microfiber by electrospinning technique, deliver this system to lung cancer cell A549 in vitro and observe the inhibition of cancer cell and to research effectiveness of controlled drugs delivered by electrospinning technique in antitumor field. Methods Lung cancer cell A549 was cultivated in vitro and incubated on 96-well plates with density of 1＆#215;104 per well. The plates were incubated at 37 ℃ and saturated humidity for 24 hours. The plates were taken out and drugs were delivered at different concentrations in each group. There were controlled groups. Plates were incubated for 48 hours. Add in MTT（20 μL/ well） and incubated for 4 hours. The medium containing MTT was discarded thoroughly and 150 μL DMSO was added, gently shaken to get a clear solution 10 ~ 15 min later. OD 490 was determined. Inhibition rate of drugs was calculated. Results Poly propylene carbonate loading paclitaxel and cisplatin controlled delivery system by electrospinning technique could inhibit cancer cell in vitro, stronger than naked paclitaxel and cisplatin and their single drug-loaded microfiber. Poly propylene carbonate loading paclitaxel or cisplatin has stronger inhibition to A549 lung cancer cells than naked paclitaxel or cisplatin. Blank poly propylene carbonate showed no inhibitory effect on the cancer cells. Conclusion Poly propylene carbonate loading paclitaxel and/ or cisplatin by electrospinning technique could inhibit lung cancer cells in vitro significantly. Controlled drug-delivery system by electrospinning technique could implant antitumor drugs locally, reduce toxicity and side effect of chemotherapeutics and have a great application potential.