不同年龄段人群蛋白质N-糖基的结构谱型研究

Comparsion of structure of protein N-glycosylation profiles between age groups

目的分析比较不同年龄人群的血浆N-糖基的结构谱型。方法采用整群抽样,以2009年4至7月在首都医科大学宣武医院进行健康体检的212名居民为研究对象,基于高效液相色谱法进行血浆糖基组检测。将年龄以中位数35为切点分组,不同年龄组的糖基含量比较采用两独立样本t检验或非参数检验。结果 212名研究对象的平均年龄为(37.78±17.89)岁,性别比为0.88。无论男性女性,半乳糖基化水平,≥35岁组二半乳糖聚糖含量较高、双触角非半乳糖聚糖含量较低;在两分支糖基的唾液酸化水平,<35岁组二唾液酸化双触角聚糖含量较高;在唾液酸化水平,≥35岁组单唾液酸含量高、三唾液酸较低;岩藻糖基化位置,≥35岁组核心岩藻糖化聚糖含量高;在分支水平,≥35岁组双触角聚糖含量较高,三触角聚糖和四触角聚糖含量较低(P≤0.03)。结论血浆N-糖基结构可能与年龄有关。

Objective To explore difference of plasma N-glycosylation structure between age groups. Methods Based on cluster sampling, 212 residents of a certain community were selected and the serum N-glycans profiles were obtained by high performance liquid chromatography（ HPLC）. Two independent samples t test or nonparametric tests were used to compare the N-glycosylation structure between age groups divided by 35. Results The average age of the 212 subjects was（37. 78±17. 89） years, and the sex ratio was 0. 88. More digalactosylated glycans, monosialylated glycans, core-fucosylated glycans and biantennary glycans were detected in 35 years or more, whereas more biantennary non-galactosylated glycans, disialylated biantennary glycans, trisialylated glycans and triantennary glycans were seen in younger than 35 years（P≤0. 03）. Conclusion The N-glycome profile in serum is age dependent. This should be taken into consideration in the development of serum glycome markers.