年龄对小鼠继发性肝泡状棘球蚴模型的影响

Age impact in mouse model of secondary hepatic alveolar echinococcus

目的探讨不同年龄对继发性肝泡状棘球蚴的影响。方法分别选取8周龄、18周龄、28周龄雌性昆明小鼠29、25、25只,用20%乌拉坦腹腔注射麻醉后,运用切开上腹部皮肤经腹壁肌层透视下肝穿刺方法分别对3组小鼠进行肝脏注射E.m组织混悬液,制备继发性肝泡状棘球蚴小鼠模型。接种后饲养100d,行安乐死并解剖。结果 3组小鼠存活率分别为:62.1%、84%、68%(P>0.05)。肝脏E.m感染率分别为:72.2%、71.4%、76.5%(P>0.05)。肝脏E.m包囊直径分别为:0.915±0.103cm、1.247±0.112cm、1.215±0.197cm(P>0.05)。肝脏E.m包囊质量分别为:0.332±0.035g、0.532±0.155g、0.382±0.085g(P>0.05),差异无统计学意义,小鼠肝E.m组织HE染色无差异。结论利用18周龄小鼠作为造模动物制作继发性肝泡状棘球蚴模型,实验小鼠存活率高。

Age impact in mouse model of secondary hepatic alveolar echinococcus was investigated in this research . Twenty-nine 8-week-old ,twenty-five 18-week-old and twenty-five 28-week-old female mice were anesthetized with 20% ure-thane by intraperitoneal injection and then transhepatically injected by Echinococcus multilocularis （E .m） tissue suspension through skin incision and abdominal muscle to liver in all three groups to establish mouse model of secondary hepatic alveolar e-chinococcus .Results showed that the survival rates for the three groups of mice were 62 .1% ,84% and 68% ,respectively （P〉0 .05） .The E .m infection rates in liver were 72 .2% ,71 .4% and 76 .5% ,respectively （P〉0 .05） .The diameter of E .m cysts in liver were 0 .915 ± 0 .103 cm ,1 .247 ± 0 .112 cm and 1 .215 ± 0 .197 cm ,respectively （P〉0 .05） .The mass of E .m cysts in liver were 0 .332 ± 0 .035 g ,0 .532 ± 0 .155 g and 0 .382 ± 0 .085 g ,respectively （P〉 0 .05） .HE stain showed no difference in pathology .Results indicated that the establishment of secondary hepatic alveolar echinococcus model by using transhepatic injection through skin incision and abdominal muscle of 18-week-old mice was capable of simplifying operation and improving the survival rate of the mice .