高频超声心动图评价阿霉素对小鼠心功能的早期影响

Early assessment of left ventricular dysfunction in mice with doxorubicin-induced cardiotoxicity by high-frequency echocardiography

目的 评价高频超声心动图对阿霉素（ADM）造模心力衰竭小鼠早期心功能减低的诊断价值,并确定ADM毒性致心功能减低的早期监测指标.方法 40只小鼠随机分为阿霉素注射组（ADM组）及对照组,每组20只,每周分别注射ADM及同等剂量生理盐水1次,连续注射5周并测定ADM组与对照组小鼠各项心功能指标,与检测的小鼠脑钠肽（BNP）值及病理结果作为金标准进行比较.结果 ①经胸高频超声心动图能清晰显示小鼠的左室长轴观及心尖四腔观;②干预5周后,观察发现两组小鼠体质量、心率、左室射血分数（LVEF）、左室短轴缩短率（LVFS）、E/A、E峰减速时间（EDT）等指标差异有统计学意义（P＜0.05）;③阿霉素注射组小鼠舒张功能的减低早于其收缩功能的减低;④BNP与LVEF、E/A呈直线负相关（r=-0.622,P＜0.05;r=-0.593,P＜0.01）,与EDT呈直线正相关（r=0.501,P＜0.05）.结论 高频超声心动图能准确对ADM毒性造成的小鼠心功能损伤进行早期评价,且与血BNP值有良好的相关性.

Objective To evaluate the diagnostic value of high frequency echocardiography on mice with doxorubicin-induced cardiotoxicity and find out the most valuable monitoring indexes for early doxorubicin-induced myocardial damage.Methods Forty mice were divided into doxorubicin-injection group （ADM group） and control group randomly.Twenty mice in each group were injected with doxorubicin and saline at the same dose for 5 weeks continuously.The parameters of cardic function of ADM group and contol group were observed by high-frequency echocardiography after infection.Brain natriuretic peptide （BNP） tested by euzymelinked immunosorbent assay（ELISA） and pathological findings were used as the golden standard.Results ①The left ventricular long axis view and the apical four chamber view could be displayed clearly by transthoracic high-frequency echocardiography.②After 5 weeks intervention,weight,heart rate,left ventricular ejection fraction （LVEF）,left ventricular fractional shortening （LVFS）,E/A,E wave deceleration time（EDT） and other parameters showed marked difference between ADM group and control group（P ＜0.05）.③The decrease of diastolic function was earlier than systolic function in ADM group.④LVEF and E/A presented good negative linear correlation to BNP（r =-0.622,P ＜0.05; r =-0.593,P ＜0.01）.EDT showed positive linear correlation to BNP（r =0.501,P ＜0.05）.Conclusions Cardic function of mice with doxorubicin-induced myocardial damage could be early evaluated accurately by high-frequency echocardiography,which contained good relativity with BNP.