雌激素及顺铂对卵巢癌细胞A2780PADI4的表达及其细胞增殖的影响

Effects of estrogen and cis-platinum on PADI4 expression and cell proliferation in ovarian cancer cell line A2780

目的探讨雌激素及顺铂对卵巢癌细胞A2780中肽酰基精氨酸脱亚胺酶4(PADI4)表达的作用及其对癌细胞增殖的影响。方法在培养的卵巢癌细胞系A2780细胞中加入不同浓度17β-雌激素作用96 h后(10-12、10-10、10-8、10-6、10-4mmol/L),采用实时定量PCR及Western Blotting检测PADI4表达,并用CCK-8试剂盒检测细胞增殖,选取PADI4的表达变化最明显的雌激素浓度,将A2780细胞分为空白对照组、雌激素组、顺铂组、雌激素加顺铂组(联合组),采用上述方法检测各组PADI4的表达和细胞的增殖情况。结果 1雌激素作用96 h后PADI4表达及细胞增殖随雌激素浓度升高而逐渐降低,但当雌激素浓度升至10-4mmol/L时PADI4表达及细胞增殖率开始升高。2与空白对照组比,联合组和顺铂组培养96 h后PADI4表达均明显降低,且联合组细胞增殖率较雌激素组低。结论雌激素可随浓度不同调控卵巢癌细胞A2780中PADI4的表达和细胞增殖;雌激素对卵巢癌细胞增殖的影响可能与PADI4表达有关。

Objective To investigate the effects of estrogen and cis-platinum on PADI4 expression and cell proliferation in ovarian cancer cell line A2780. Methods A2780 ceils were cultured in medium with estrogen （ 10^-12, 10^-10, 10^-8, 10^-6, 10^-4mmol/L）. The expression of PADI4 was detected using real-time PCR and Western blotting after 96 hours. The cell proliferation was measured with CCK-8 assay. The concentration of estradiol-17β was chosen at which PADI4 expression changed the most significantly. A2780 ceils were grouped into blank control group, single-agent estra- dio1-17β group, single-agent cis-platinum group and estradiol-17β plus cis-platinum group （combination group）. The PADI4 expression and cell proliferation were measured by real-time PCR, Western blotting and CCK-8 assay. Results ① The PADI4 expression and cell proliferation decreased in A2780 cells treated with estradiol-17β in the range of 10^-12 mmol to 10^-6mmol, but increased with higher concentration of estradiol-17β. A significant association was detected be- tween PADI4 expression and cell proliferation. ② Compared with the blank control group, PADI4 expression decreased in cells cultured with estradiol-17β plus cis-platinum and single-agent cis-platinum group, and the proliferation of combination group was lower than that of the single-agent cis-platinum group. Conclusion Estradiol-17β regulates the expression of PADI4 and cell proliferation of ovarian cancer. The role of estradiol-17β may be associated with PADI4 expression.