植物甾醇酯对高脂饮食诱导的非酒精性脂肪肝的预防作用

Preventive effects of phytosterol ester on high fat diet-induced non-alcoholic fatty liver disease in rats

目的:研究植物甾醇酯(phytosterol ester,PSE)对高脂饮食诱导的非酒精性脂肪肝(nonalcoholic fatty liver disease,NAFLD)大鼠的预防作用.方法:通过高脂饮食诱导建立NAFLD大鼠模型,饲喂高脂饮食的同时灌胃给予不同剂量PSE[低剂量组:0.05 g/100 g体质量(body weight,BW);中剂量组:0.10 g/100 g BW;高剂量组:0.15 g/100 g BW],观察其对大鼠血脂、肝脂的影响.结果:与模型组(high-fat diet group,HF)相比,低剂量PSE组(low dosage of PSE treatment group,PSE L)血清低密度脂蛋白胆固醇(lowdensity lipoprotein cholesterol,LDL-C)降低13.6%,剂量增加未表现出更明显的降低效果;各PSE干预组肝脏甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、游离脂肪酸(free fatty acids,FFA)含量均显著低于HF组,其中PSE L的FFA接近正常对照组(normal controlled group,NC)水平.此外,中剂量PSE干预组(medium dosage of PSE treatment group,PSE M)、高剂量PSE干预组(high dosage of PSE treatment group,PSE H)血糖水平显著低于HF组,与正常对照组接近.HE病理染色结果显示,PSE能明显减轻肝脏脂肪变性程度.结论:PSE能在一定程度上纠正高脂饮食诱导的血脂、血糖代谢异常,减少肝脂质积累,显著改善肝脏脂肪变性程度,对高脂饮食诱导的NAFLD有良好的预防作用.

AIM： To explore the preventive effects of phytosterol ester（PSE） on high fat diet-induced nonalcoholic fatty liver disease（NAFLD）.METHODS： An NAFLD rat model was established by giving a high fat diet, and different dosages of PSE [PSEL： 0.05 g/100 g body weight（BW）; PSEM： 0.10 g/100 g BW; PSEH： 0.15 g/100 g BW] fortified milk were given to rats by intragastric administration. Serum lipid profile and liver lipids were detected, and histological changes were observed after HE staining.RESULTS： Compared with the high fat diet group（HF group）, serum low-density lipoprotein cholesterol（LDL-C） in animals in the PSEL group was lowered by 13.6%, while no further effect was observed with higher concentrations of PSE; liver triglyceride（TG）, total cholesterol（TC）, and free fatty acids（FFAs） were reduced significantly in all the PSE treatment groups, among which the level of FFA was close to the normal level. Glucose（GLU） levels in the PSEM and PSEH groups were lower than that in the HF group, which were close to the normal level. HE staining results showed that the degree of hepatic steatosis was obviously ameliorated in all the PSE treatment groups.CONCLUSION： PSE could effectively reduce serum LDL-C and hepatic fat accumulation induced by a high fat diet, and attenuate hepatic steatosis, thus exhibiting preventive effects on NAFLD.