动脉粥样硬化患者外周血CD4 T细胞及CD4^+CD25^(high)CD127^-调节性T细胞的变化

Alterations of CD4 T cells and CD4^+CD25^(high)CD127~– regulatory cells in patients with arteriosclerosis

目的:探讨动脉粥样硬化(AS)患者外周血CD4 T细胞及调节性T细胞(CD4+CD25high CD127-Treg)比例及功能的变化。方法:选择AS患者(AS组)及健康体检者(健康对照组)各40例,抽取外周静脉血,用流式细胞术测定CD4 T细胞、CD8 T细胞、IFN-γ+CD4 T细胞、IFN-α+CD4 T细胞和CD4+CD25high CD127-Treg细胞比例,以及各自的CD4+CD25high CD127-Treg细胞与CD4 T细胞共培养后,CD4 T细胞增殖能力以及CD4 T细胞分泌炎症因子能力的变化。结果:与健康对照组比较,AS组外周血CD4 T细胞比例及CD4 T/CD8 T比值明显升高、IFN-γ+CD4T及IFN-α+CD4 T细胞比例明显升高(均P<0.05),而CD8 T细胞比例与CD4+CD25high CD127-Treg细胞比例差异无统计学意义(均P>0.05)。健康对照组CD4 T细胞的增殖能力、IFN-γ及IFN-α分泌能力在与CD4+CD25high CD127-Treg细胞共培养后较其单独培养均明显降低(均P<0.05),而AS组CD4 T细胞的以上指标在与CD4+CD25high CD127-Treg细胞共培与单独培养间差异均无统计学意义(均P>0.05)。结论:AS患者外周血CD4+CD25high CD127-Treg细胞功能的丧失,导致外周血CD4 T细胞比例升高、分泌炎症因子能力增强,从而引发AS的炎症状态。

Objective： To investigate the changes in proportion and function of the CD4 T cells and regulatory cells（CD4＋CD25high CD127– Treg cells） in peripheral blood of arteriosclerosis（AS） patients.Methods： AS patients（AS group） and healthy subjects undergoing health maintenance examination（healthy control group） were selected with 40 cases in each group, and then the samples of peripheral venous blood were obtained. Using l ow cytometry, the proportions of CD4 T cells, CD8 T cells, IFN-γ＋ CD4 T cells, TNF-α＋CD4 T cells and CD4＋CD25high CD127– Treg cells were measured, and the changes in ability of proliferation and cytokine secretion of the CD4 T cells after co-culture with respective CD4＋CD25high CD127– Treg cells were also determined.Results： In AS group compared with healthy control group, the proportion of CD4 T cells and CD4 T/CD8 T ratio were significantly increased and the proportions of IFN-γ＋ CD4 T cells and IFN-α＋ CD4 T cells were also significantly increased（all P〈0.05）, but the proportions of CD8 T cells and CD4＋CD25high CD127– Treg cells showed no significant difference（both P〉0.05）. In healthy control group, the abilities of proliferation and IFN-γ and IFN-α secretion in CD4 T cells were all significantly lower in co-culture with CD4＋CD25high CD127– Treg cells than those in single culture（all P〈0.05）, while these parameters in AS group showed no significant difference between single culture and co-culture（all P〉0.05）.Conclusion： There is loss of function of CD4＋CD25high CD127– Treg cells in AS patients, which results in the increased proportion and cytokine secretion ability of CD4 T cells, and thereby triggers the inflammatory state in AS.