木糖醇口香糖咀嚼时间对口腔微生物群落的影响

Effects of chewing xylitol-containing gum on oral microflora

目的：研究咀嚼木糖醇口香糖时间长短对口腔常见细菌的影响。方法：纳入60名年龄在18～22岁口腔健康志愿者，随机分为3组（n＝20）。 A组于午餐后咀嚼木糖醇口香糖45 min；B组于午餐后咀嚼等剂量同品牌木糖醇口香糖15 min；C组（对照组）午餐后不咀嚼口香糖。于咀嚼口香糖3周后收集唾液，接种于BHI、MSB、Rogosa培养基平板，计数总菌落与主要条件致龋菌的数量，评估咀嚼口香糖时间长短对口腔微生物群落的影响。结果：咀嚼木糖醇口香糖组口腔微生物水平低于对照组（P＜0．05）），但咀嚼口香糖时间长短对口腔微生物水平的影响无统计学差异。结论：咀嚼口香糖15 min即可达到良好口腔清洁效果，延长咀嚼时间并不能显著增强其对龋病相关微生物的清除作用。

AIM：To investigate the effects of chewing xylitol-containing gum on the microbial composition in oral cavity.METHODS：60 volunteers aged 18-22 years were included and randomly divided into 3 groups （ n=20）：45 min chewing after lunch （group A）, 15 min chewing after lunch （group B） and non-chewing control （group C） .Saliva was collected on day 21 and cultured on BHI, MSB and Rogosa agar plates for colony enumeration.RE-SULTS：Chewing xylitol-containing gum significantly decreased the level of total bacteria in saliva.However, no sig-nificant difference was observed between different duration of gum chewing.CONCLUSION：Chewing xylitol-contai-ning gum for 15 min is sufficient to suppress oral bacteria, further prolonging the duration of chewing may not necessari-ly enhance the inhibitory effect on oral microflora.