内皮祖细胞与骨折愈合:培养与移植中的问题

Endothelial progenitor cells in fracture healing: problems about culture and transplantation

背景:近年来,人们意识到血供可能是影响骨折愈合的一个因素,基于内皮祖细胞具有良好的血管化能力,将其应用于临床促进骨折愈合具有良好的应用前景。目的:介绍内皮祖细胞在骨折愈合中的研究情况。方法:以"内皮祖细胞、骨折、血管化、血管形成"为中文关键词,以"endothelial progenitor cell,fracture,neovascularization,angiogenesis"为英文关键词,采用计算机检索1980年1月至2014年9月CNKI数据库、万方数据库和Pub Med数据库相关文章,选择48篇内皮祖细胞与骨折愈合血管化相关的文章进行分析。结果与结论:大量的研究证明内皮祖细胞具有血管化能力以及促进骨折愈合的能力,因此将内皮祖细胞应用于组织工程支架可能会增强支架在体内的存活率,从而挽救一大部分骨折延迟愈合与不愈合患者。但是对于临床来说,使用内皮祖细胞进行大规模治疗仍然存在许多问题,如内皮祖细胞体外分离培养、扩增,移植细胞数量以及移植细胞所需要的支架材料的选择等,这些问题的解决依赖于更进一步的研究。

BACKGROUND：With the development of biochemistry and cel biology, fracture has being study deeper, blood supply has been known to be an important factor influencing the fracture healing. Endothelial progenitor cel s with good ability of angiogenesis wil have a good clinical prospect in fracture healing. OBJECTIVE：To review the recent research of endothelial progenitor cel s in fracture healing. METHODS：A computer-based online search of CNKI, Wanfang, PubMed databases was performed to col ect articles published between 1980 and 2014 with the key words“endothelial progenitor cel , fracture, neovascularization, angiogenesis”in Chinese and English. A total of 48 articles addressing endothelial progenitor cel for angiogenesis in fracture healing were included in result analysis. RESULTS AND CONCLUSION：Increasing evidence has shown that endothelial progenitor cel s have great ability of neovascularzition and angiogenesis. Endothelial progenitor cel s used in tissue engineering scaffolds can promote the survival rate of scaffolds in vivo, which is appropriate to a great part of delayed union and nonunion patients. However, the large-scale treatment with endothelial progenitor cel s stil has many problems, such as isolation, culture and amplification of endothelial progenitor cel s in vitro, the number of transplanted cel s and selection of scaffolds for transplanted cel s, which need further research.