人类疾病干细胞移植实验:基础研究现状及进展

Stem cell transplantation for human diseases: current status and progress in basic research

背景:随着干细胞培育技术的进步和发展,干细胞移植已经成为现今生物细胞学研究的热点之一。目的:对国内外开展的人类难治性疾病干细胞移植基础研究的现状及新进展作一综述。方法:在标题和摘要中,以"induced pluripotent stem cells,neural stem cells,top-up between stem cells,transplantation"或"干细胞,再生,移植"为检索词,检索中国期刊全文数据库(CNKI)、维普数据库、万方数据库和Pub Med数据库中2008年1月至2014年10月关于干细胞移植动物实验的文章。同一领域文章则选择近期发表或发表在权威杂志上的文章。初检得到113篇文献,根据纳入标准,选择其中的50篇文献进行综述。结果与结论:随着干细胞培育技术的进步,人类疾病的干细胞移植试验基础研究得到了推动和开展,并已取得了阶段性成果,这就为下一步更广泛、更深入地基础研究创造了条件。相信随着干细胞移植基础研究一系列难点问题的解决,将为人类疾病,尤其是难治性疾病的治疗带来希望。

BACKGROUND：With the development of stem cel culture technology, stem cel transplantation has been a research hotspot in the biological cytology. OBJECTIVE：To review the current situation and progress of basic research on stem cel transplantation for human refractory diseases. METHODS：A computer-based search of CNKI, VIP, Wanfang and PubMed databases was performed for articles related to animal experiments of stem cel transplantation published from January 2008 to October 2014. The key words were“induced pluripotent stem cel s, neural stem cel s, top-up between stem cel s, transplantation”in Chinese and English in the title and abstract. Papers published in authoritative journals or recently were preferred. Total y 113 articles were searched initial y, and only 50 articles were included in result analysis. 〈br〉 RESULTS AND CONCLUSION：With the progress of stem cel culture technology, basic research on the stem cel transplantation for human diseases have been promoted and carried out, and these researches have achieved phased outcomes that provide conditions for more extensive and in-depth fundamental research. It is believed that with the development of basic research on stem cel transplantation to solve a series of difficult problems, stem cel transplantation wil bring hope for treatment of human disease, especial y for treatment of refractory disease.