摘要
目的研究分析不同浓度银屑病患者真皮间充质干细胞(Dermis mesenchymal stem cells,DMSCs)对人永生化角质形成细胞(Ha Ca T)的作用。方法取银屑病患者和正常人的真皮,获得纯度高的DMSCs,将Ha Ca T在Transwell小室下室接种,同时按照第5代DMSCs与Ha Ca T细胞之间的比例(1∶1、5∶1、10∶1)将DMSCs分3组接种在Transwell上室,并设正常对照组和自然增殖组,采用实时细胞分析系统(RTCA)对Ha Ca T细胞进行增殖检测,培养74 h后用细胞计数法检测Ha Ca T细胞的数量。结果共培养组较自然增殖组Ha Ca T细胞计数减少,与健康对照组相比,银屑病患者组Ha Ca T细胞计数减少不明显,以银屑病患者DMSC与Ha Ca T细胞按照1∶1共培养组为著。结论 1DMSCs对Ha Ca T细胞增殖有抑制作用。2与正常对照组、自然增殖组和其他比例组相比银屑病患者DMSCs与Ha Ca T 1∶1共培养组对Ha Ca T细胞增殖抑制作用减弱更显著。
Objective To investigate the effects of different doses of human dermis mesenchymal stem cells （DMSCs） on HaCaT cell in patients with psoriasis lesions in co-culture system. Methods DMSCs were isolated from healthy male and patients with psoriasis vulgaris, and subjected to primary culture. Culture HaCaT cells in the co-culture system with DMSCs of passage five （DMSCs one times in HaCaT, DMSCs five times in HaCaT, DMSCs 10 times in HaCaT） were obtained from psoriatic skin lesions and healthy human skins. The proliferation of HaCaT cell was investigated in a co-culture system by real-time cell analysis system and cell counting. Results Different concentrations of DMSCs derived from psoriatic skin lesions had weaker inhibitory effect on keratinocytes proliferation, compared with healthy controls. Co-culture of DMSCs and HaCaT cells at 1：1 radio gave the most obvious results. Conclusion The results suggest that DMSCs inhibited the proliferation of HaCaT ceils obviously. The inhibitive effect of the DMSCs one times in HaCaT group on HaCaT proliferation had a drastic decrease, compared with other groups.
出处
《中国中西医结合皮肤性病学杂志》
CAS
2014年第6期352-356,共5页
Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine
