大麦DH矮杆突变体的表型分析及其对外源赤霉酸的响应

Phenotypic Analysis and Gibberellic(GA_3) Response of Barley Double Haploid Dwarf Mutants

利用物理诱变结合小孢子培养创造新的变异可以为作物遗传改良提供新的遗传资源。本研究利用60Coγ-射线辐照优良大麦品种‘花30’和‘花11’干种子,取其植株上花药,游离小孢子培养获得3份纯合的矮杆突变体。突变体的株高为亲本的52.4%~61.1%,株高降低效应显著。苗期以及拔节期赤霉酸处理表明突变体及野生型均为赤霉酸敏感型,而且不同突变体对赤霉酸反应的敏感性不同。

Physical mutagenesis combined with microspore culture can create the germplasm with new varia- tion for crop improvement. In this study, three homogeneous dwarf mutants of two barley cultivars ＇Hua30＇ and ＇Huall＇ were obtained through isolated microspore culture on the plants derived from 60Co ）,-rays irradiated seeds. The dwarf mutants showed a significant reduction on plant height, which was 52.4%-61. 1% of the wild types. Both the mutants and their wild types were sensitive to exotic GA3 treatment at seedling and elongation stages, however, the mutants revealed different sensibility to GA3.