3种化学物对血脑屏障通透性影响的体内、外实验对比研究

Experimental study of the effect of three chemicals on blood-brain barrier (BBB) permeability in vivo and in vitro

目的观察分析乙酸铅、硫酸铝和甲醛3种化学物对血脑屏障(blood-brain barrier,BBB)通透性的作用影响。方法利用血脑屏障模型(blood-brain barrier model,BBBM)进行3种化学物通透性试验,计算通透系数(Permeability Coefficient,Pe)用以反映血脑屏障通透性变化情况;伊文氏蓝比色法测定3种化学物对Wistar试验大鼠血脑屏障通透性作用影响。对体内、外测试结果一致性进行比较分析。结果求得乙酸铅、硫酸铝和甲醛3种化学物Pe值分别为(2.38±0.54)×10-3、(2.07±0.33)×10-3和(1.11±0.28)×10-3cm/min,其中乙酸铅和硫酸铝组Pe值与对照组[(1.06±0.17)×10-3cm/min]比较,差异具有统计学意义(P<0.01);动物试验结果显示,乙酸铅、硫酸铝和甲醛对试验大鼠大脑皮质血脑屏障通透性影响增高和对照组比较差异均有统计学意义(P<0.05)。结论乙酸铅和硫酸铝两种化学物体内外均能对血脑屏障通透性产生显著影响。甲醛在体内可对血脑屏障通透性产生显著影响。甲醛在体外对血脑屏障通透性产生影响有待进一步证实。

Objective To observe the effect of lead acetate, aluminum sulfate and formaldehyde on blood-brain barrier （BBB） permeability. Methods Permeability coefficient （Pe） of three chemicals was calculated through in vitro permeability test utilizing blood- brain barrier model （BBBM）. Evens Blue （EB） colorimetric method was used to evaluate the impact of three chemicals to the BBB permeability of Wistar rat. Consistency of in vivo and in vitro test results was comparatively analyzed. Results Pe values of lead acetate, aluminum sulfate and formaldehyde are calculated （2.38 ±0.54） × 10 -3,（2.07 ±0.33） × 10 -3 and （1.11±0.28） × 10^-3 cm/min respectively. Among them, the Pe values of lead acetate and aluminum sulfate were statistically higher than that of the control group[ Pe value of the control group was （1.06 ± 0. 17）× 10^-3 cm/min] （P 〈 0.01 ）. Animal test indicated that the impact of lead acetate, aluminum sulfate and formaldehyde on BBB of mice cerebral cortex permeability had statistical significance compared with the control group （P 〈 0. 05 ）. Conclusion Lead acetate and aluminum sulfate can increase BBB permeability both in bith and in vitro test. As to formaldehyde, a future in vitro research on its effect on BBB is necessary despite the significant effect on BBB permeability in vivo test.