HPLC法测定金沙藤中绿原酸、咖啡酸和p-香豆酰葡萄糖

Simultaneous determination of chlorogenic acid,caffeic acid,and 6-O-p-coumaroyl-D-glucopyranose in Lygodii Herba by HPLC

目的建立一种同时测定金沙藤中绿原酸、咖啡酸和p-香豆酰葡萄糖的方法。方法采用HPLC法,色谱柱为Phenomenex Cl8（250 mm×4.6 mm,5μm）,甲醇-乙腈-1%冰醋酸（5∶5∶90）为流动相,检测波长为325 nm,柱温35℃。结果绿原酸、咖啡酸和p-香豆酰葡萄糖分别在0.368-2.760、0.064-0.480、0.102-0.765μg内呈良好的线性关系,平均加样回收率分别为101.48%、99.56%、101.73%,RSD分别为1.44%、0.92%、1.62%。结论该方法操作简便、准确、重复性好,可用于金沙藤及其提取物的质量控制。

Objective To establish a method for the determination of chlorogenic acid,caffeic acid,and 6-O-p-coumaroyl-Dglucopyranose in Lygodii Herba.Methods The determination was developed on Phenomenex C18 column（250 mm × 4.6 mm,5 μm）,methanol-acetonitrile-1% glacial acetic acid（5︰5︰90） was used as mobile phase,the detective wavelength was 325 nm,and the column temperature was 35 ℃.Results The linear ranges of chlorogenic acid,caffeic acid,and 6-O-p-coumaroyl-D-glucopyranose were 0.368—2.760,0.064—0.480,and 0.102—0.765 μg,respectively.The average recoveries were 101.48%（RSD = 1.44%）,99.56%（RSD = 0.92%）,and 101.73%（RSD = 1.62%）,respectively.Conclusion The method is simple,accurate,and highly reproducible,which could be used for the quality control of Lygodii Herba.