3种甲苯胺蓝异染肥大细胞方法的比较

The comparison of three different methods of toluidine blue metachromatic for mast cells

目的比较3种甲苯胺蓝异染肥大细胞方法的应用性。方法采用甲苯胺蓝水溶液与高锰酸钾配比染色(方法一)、甲苯胺蓝酒精溶液和氯化钠溶液配比(方法二)、甲苯胺蓝水溶液染色、冰醋酸分色(方法三)3种方法对人和小鼠肺组织冰冻切片进行染色,比较染色结果、并评价方法稳定性及实用性。结果方法一染色肺组织切片,结果切片蓝色背景清晰,肥大细胞胞质内紫红色颗粒鲜明,与背景蓝色对比度高,易辨识,且多次重复染色效果稳定;方法二及方法三肺组织的蓝染背景、和肥大细胞胞浆颗粒的紫红色染之间的对比度均不及方法一清晰,稳定性亦次之。结论甲苯胺蓝水溶液与高锰酸钾配比染色法异染肺组织冰冻切片,结果清晰、操作简便且稳定性好,适合在相关检验中选用。

Objective This study tries to compare the application of three different methods to dye mast cells with toluidine blue. Methods The aqueous solution of toluidine blue and potassium permanganate（ method1） 、the alcoholic solution of toluidine blue and aqueous solution of sodium chloride（ method2） 、the aqueous solution of toluidine blue and colour separation with glacialaceticacid（ method3）. The frozen sectionof human＇s and mouse＇s lung tissue were stained with three different solution respectively. Then comparing the dyeing effects, practicability and stability of three methods. Results Clearer blue background,higher mauve granules background contrast and more stable dyeing effect had been observed on the frozen section when using method1,compared with using method2 and method3. Conclusion The method1 is a rapid,economic,stable and high positive rate dyeing method which can be used to the relevant detection.