摘要
目的:探讨促肝细胞再生磷酸酶1(phosphatase of regenerating liver cell-1,PRL-1)对舌癌细胞侵袭能力的影响及其可能的机制。方法:应用si RNA-PRL-1慢病毒载体转染处理舌癌TCA8113细胞株后,分别采用荧光定量PCR和蛋白质印迹检测PRL-1基因和基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)m RNA和蛋白水平;采用Transwell小室模型实验检测癌细胞的侵袭能力。结果:si RNA-PRL-1慢病毒转染组癌细胞PRL-1在RNA(0.425 0±0.010 0)和蛋白(2.720 0±0.110 0)水平明显下调(P=0.000,P=0.000),同时MMP-2及MMP-9在RNA(0.562 2±0.180 0,0.617 1±0.100 0)及蛋白(0.592 4±0.010 0,0.476 2±0.020 0)表达水平降低(P=0.024,P=0.010,P=0.000,P=0.000);Transwell实验si RNA-PRL-1慢病毒转染组细胞通过数(64.33±4.04)明显减少(P=0.000)。结论:si RNA-PRL-1转染可抑制舌癌细胞侵袭,其机制可能与PRL-1基因下调MMP-2及MMP-9的表达有关。
Objective :To study the effects of phosphatase of regenerating liver cell-1(PRL-1 ) small interfering RNA(siRNA)on invasion of tongue cancer cells(TCA8113) and the mechanism. Methods:after tongue squamous cancer cell line TCA8113 being trans- fected by PRL-1 siRNA,the mRNA and protein of PRL-1 and matrix metalloproteinase-2(MMP-2) and matrix metalloproteiuase-9 (MMP-9) were determined by real-time PC R and Western blot assay, respectively. TCA8113 cell invasiveness was detected through Transwell chamber using model test. Results :After transfection by PRL-1 siRNA,the level of mRNA(0.425 0 ± 0.010 0) and protein (2.720 0 ± 0.110 0) of PRL-1 was down-regulated(P=0.000,P=0.000). The mRNA level of MMP-2 and MMP-9(0.562 2 ± 0.180 0, 0.617 1±0.100 0)as well as the protein level of MMP-2 and MMP-9(0.592 4 ±0.010 0,0.476 2 ± 0.020 0) were decreased obviously (P=-0.024,P=0.010, P=0.000,P=0.000). The invasion ability of human tongue squamous cancer cell was reduced (64.33 ± 4.04)(P= 0.000). Conclusion :PRL-1 gene might play an important role in development of human oral squamous cell carcinoma and down- regulation of PRL-1 could inhibit invasion of human tongue squamous cancer cell. This may be related to the lower expression of MMP-2 and MMP-9.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2014年第11期1622-1626,共5页
Journal of Chongqing Medical University
基金
重庆市重点课题资助项目(编号:2009-1-33)
重庆市卫生局重点资助项目(编号:2013-1-030)
关键词
舌癌
侵袭
促肝细胞再生磷酸酶-1
基质金属蛋白酶
tongue squamous cancer
invasion
phosphatase of regenerating liver cell- 1
matrix metalloproteinase