IL-33/ST2信号通路在比索洛尔治疗舒张性心力衰竭中的作用

IL-33/ST2 signaling pathway's role in the treatment of diastolic heart failure by using bisoprolol

目的观察IL-33/ST2信号通路在比索洛尔治疗舒张性心力衰竭(DHF)中的作用。方法 24只舒张性心力衰竭老鼠随机分为对照组(n=12)及比索洛尔组(n=12),分别用蒸馏水及比索洛尔溶液治疗,疗程6周。观察2组的左室压力、左室充盈时间(LVFT)、左室舒张压、等容收缩期左心室内压最大上升速率及心肌细胞ST2蛋白及血清肿瘤坏死因子(TNF-α)的浓度。结果比索洛尔组小鼠左室收缩压、左心室平均压均显著低于对照组(P<0.05),但2组左室舒张压差异无统计学意义(P>0.05)。比索洛尔组等容收缩期左心室内压最大上升速率显著高于对照组(P<0.05);等容舒张期左心室内压最大下降速率显著低于对照组(P<0.05),而左室充盈时间2组差异无统计学意义(P>0.05)。比索洛尔组IL-33蛋白表达显著降低(P<0.05),但ST2表达量和血清中TNF-α浓度2组差异均无统计学意义(P>0.05)。结论比索洛尔可有效降低舒张性心力衰竭小鼠的左室血压,改善左心室顺应性,其机制可能与心肌细胞内IL-33蛋白表达量显著减少有关。

Objective To evaluate the IL-33/ST2 signaling pathway′s role in the treatment of diastolic heart failure （ DHF） by using bisoprolol.Methods Twenty four diastolic heart failure rats were randomly divided into control group （ n=12 ） which were given distilled water and the biso-prolol group （ n=12） which were given solution of bisoprolol treatment.The treatment period lasted for 3 months.The data of left ventricular pressure , left ventricular filling time （ LVFT） , left ventricular end-dias-tolic pressure , the maximum rate of isovolumic left ventricular systolic pressure rise were observed in the two groups.The cardiomyocytes ST2 protein and expression of serum TNF -αwere compared.Results The left ventricular systolic pressure and left ventricular mean pressure were significantly lower in bisoprolol group than that in control group （ P 0.05 ）.The maximum rate pressure rise of left ven-tricular systolic content in bisoprolol group was significantly higher than that in the control group （ P0.05 ）.Conclusion Bisoprolol may reduce left ventricular blood pressure in diastolic heart failure mice , and im-prove left ventricular compliance , which may be associated with a significant reduction in myocardial cells IL -33 pro-tein expression.