摘要
目的用CDAP活化b型流感嗜血杆菌(Hib)荚膜多糖,制备b型流感嗜血杆菌荚膜多糖及D蛋白疫苗。方法采用CDAP法在不同p H值条件下活化Hib荚膜多糖,再通过乙二酰肼(ADH)与D蛋白共价结合,制备Hib荚膜多糖与其D蛋白结合物原液,并对三批结合物原液的各项指标进行检测。获得的结合物原液免疫BALB/c小鼠,应用ELISA法检测血清中的Ig G抗体水平,评价其免疫原性。结果三批结合物原液以上检测指标均符合药典要求,衍生物中ADH含量对结合物的免疫原性有重要影响。结论用CDAP活化工艺制备的Hib荚膜多糖-D蛋白结合物适宜制备结合疫苗,为以D蛋白为载体的Hib结合疫苗的制备及五价联苗的研制奠定实验基础。
Objective To prepare haemophilus influenzae type b (Hib) capsular polysaccharide-D protein conjugate vaccine by activation of polysaccharide with 1-cyano-4-dinmethylaminopyridinium tetrafluoroborate (CDAP).Methods CDAP was used to activate the purified Hib capsular polysaccharide antigens under different level of pH.Though oxalyl hydrazine (ADH),Hib capsular polysaccharide binds covalently to D protein.The glycoprotein conjugations of three runs were tested successively on different indexes.The BALB/c mice were immunized with these conjugations.The level of IgG in serum was analyzed by ELISA.Results The above indexes of three glycoprotein conjugations conformed to the requirements of the pharmacopoeia.ADH content in activated polysaccharide had important influence on immunogenicity of glycoprotein conjugations.Conclusion Hib capsular polysaccharide was activated,and t was bonded with carrier D protein.The polysaccharide derivatives and combination concentrate were measured and their quality according with the requirement of Chinese Pharmacopoeia 2010 edition.D protein works as the carrier protein of the conjugate vaccine preparation,and makes a good basis for five league vaccine in the future.
出处
《医学研究杂志》
2014年第12期22-25,共4页
Journal of Medical Research
基金
国家高技术研究发展("863"计划)项目(2012AA02A404)
科技部国际合作项目(2010DF32890)
关键词
B型流感嗜血杆菌
荚膜多糖
D蛋白
结合疫苗
Haemophilus influenzae type b
Capsular polysaccharide
D protein
Conjugate vaccine