柯萨奇病毒A16型YY157株在Vero细胞中培养与增殖特性的研究

Characteristion of Coxsackievirus A16 strain YY157 cultured in vero cells

目的 探索柯萨奇病毒A16型（CVA16） YY157株在非洲绿猴肾（Vero）细胞中培养与增殖的合适条件.方法 把CVA16 YY157株接种于Vero细胞适应性传代,挑斑纯化后,在不同条件下培养并比较其对病毒滴度的影响.结果 CVA16 YY157株在Vero细胞中培养能导致细胞病变（CPE）,可形成蚀斑.将此CVA16病毒以0.01 ～0.1MOI接种于Vero细胞,并在低于2％牛血清浓度的培养基,35℃培养60 h,CPE可达90％以上;此条件下收获培养液上清,可得到较高滴度的病毒.结论 初步建立了CVA16 YY157株在Vero细胞中培养与增殖的方法,为其下一步的大规模培养及疫苗制备奠定了基础.

Objective To explore optimal culture conditions for proliferation of Coxsackievirus 16 （CVA16） strain YY157 in vero cells.Methods CVA16 strain YY157 strain was inoculated into vero cells and adapted by passage culturing.The proliferation condition of CAV16 was investigated after plaquepurified.Results CVA16 strain YY157 could induce cytopathic effect （CPE） and visible plaques in vero cell culture.After inoculating the virus at a MOI of 0.01-0.1 into vero cells and cultured for 60 h at 35 ℃ in medium containing less than 2% bovine serum,more than 90% cells showed CPE.The culture supernatant was harvested and a relatively high titer of CVA16 could be obtained.Conclusion A procedure for incubation and proliferation of CVA16 strain YY157 in vero cells was established,which provides a basis for its large-scale culture and vaccine development.