高脂肪饮食对大鼠青春期启动影响的实验研究

Effect of High Fat Diet on the Onset of Puberty of Rats

目的观察高脂肪饮食对大鼠青春期启动的影响。方法 20只雌性SD大鼠按配对设计的分组原则,分为标准组、高脂组,各20只。实验第1天起标准组大鼠喂养标准饲料,高脂组大鼠喂养高脂肪饲料。每天观察大鼠阴道口开放情况,当高脂组大鼠全部出现阴道口开放,即于当日下午处死全部大鼠。观察两组大鼠阴道口开放时间(VO)及第一性周期时间(D1),计算子宫、卵巢指数,采用放射免疫法检测血清黄体生成素(LH)的含量。结果与标准组比较,高脂组阴道口开放时间(VO)(33.2±1.87)天,第一动情间期时间(D1)(35.7±1.16)天,均较标准组的(36.4±0.96)天、(37.5±0.53)天明显提前(P<0.01),LH含量高脂组(6.275±0.422)m IU/m L,标准组(4.979±0.587)m IU/m L,两组比较,差异有统计学意义(P<0.05)。结论高脂肪饮食可促使大鼠青春期启动提前。

Objective To investigate the effect of high fat diet on the onset of puberty of rats. Methods Twenty female SD rats were randomized into standard group and high-fat group by paired design, 10 for each group.From the first day of the experiment, rats in standard group were fed with standard diet, rats in high-fat group were fed with high fat diet. When all 10 rats in high-fat group had vaginal opening（VO）, then they were sacrificed on the afternoon of that day. The times of VO and first cycle（D1） were observed, the uterus index and ovarian index were calculated, and the content of serum luteinizing hormone（LH） was detected by radioimmunoassay.Results Compared with standard group, VO and D1 was significantly earlier in high-fat group（VO：33.2±1.87 d vs36.4±0.96d;D1：35.7±1.16 d vs 37.5±0.53d;all P〈0.01）,and the serum LH were statistically increased in high-fat group（6.275±0.422 m IU/m L vs 4.979±0.587 m IU/m L,P〈0.05）. Conclusion The high fat diet can induce early puberty starts in rats.