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CRISPR-Cas9技术在干细胞中的应用 被引量:5

Progress of CRISPR-Cas9 in stem cell research
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摘要 成簇规律间隔短回文重复序列系统(Clustered regularly interspaced short palindromic repeats,associated RNA guided endonuclease Cas9(CRISPR-Cas9)是细菌或古细菌在长期演化过程中形成的抵御外来遗传物质的一种获得性免疫防御机制,其中II型CRISPR-Cas系统依赖Cas9核酸内切酶靶向剪切外源DNA。Cas9内切酶在向导RNA的指导下靶向性地剪切特定基因位点,已被广泛应用在不同种属的基因编辑研究中。利用CRISPR-Cas9基因编辑系统的优势,结合现有干细胞研究技术,在小鼠、大鼠,甚至灵长类动物的功能基因组研究中,可以大幅提高各种基因修饰动物的获得效率,缩短获得的时间,从而快捷有效地研究基因功能;同时,可以建立包括灵长类疾病模型在内的多种动物疾病模型,促进生物医学的发展,造福人类。 Clustered regularly interspaced short palindromic repeats associated RNA guided endonuclease Cas9 (CRISPR-Cas9) is a kind of adaptive immune defense system, which p6rotects the bacteria or archaea against foreign genetic material. Type II CRISPR-Cas system relies on Cas9 endonuclease that cuts exogenous DNA. Cas9 endonuclease targets and attacks the specific gene loci under the guidance of single guide RNA (sgRNA). CRISPR- Cas9 genome-editing technology has been widely used in different species. The application of CRISPR-Cas9 technology in stem cell research can greatly promote the generation of various genetically modified animal models from mammalians, such as mice, rats and primates, which can be used to identify the function of different genes at organismal level. Meanwhile, animal disease models can also be established efficiently, which can be applied to development of new treatment strategies.
出处 《生命科学》 CSCD 2015年第1期93-98,共6页 Chinese Bulletin of Life Sciences
基金 国家杰出青年科学基金项目(31225017) 国家自然科学基金重大研究计划集成项目(91319310)
关键词 CRISPR-Cas9技术 sgRNA 基因编辑 成体干细胞 精原干细胞 单倍体胚胎干细胞 CRISPR-Cas9 sgRNA gene editing adult stem cells spermatogonial stem cells haploid embryonic stem cells
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  • 1胡玉鸿.个人的独特性与人的尊严之证成[J].法学评论,2021(2):39-54. 被引量:21
  • 2邱仁宗.人类基因工程和对未来时代的责任(上)[J].医学与哲学,1996,17(6):284-287. 被引量:7
  • 3http://www.who.inffcardiovascular_diseases/en/.
  • 4Jennings GL, McMullen JR. Left ventricular hypertrophy: Beyond the image and defining the human cardiac phenotype in hypertension. J Hypertens 2007, 25(5): 941-7.
  • 5Chien KR, Domian I J, Parker KK. Cardiogenesis and the complex biology of regenerative cardiovascular medicine. Science 2008, 322(5907): 1494-7.
  • 6Bu L1, Jiang X, Martin-Puig S, Caron L, Zhu S, Shao Y, et al. Human ISL1 heart progenitors generate diverse multipotent cardiovascular cell lineages. Nature 2009, 460(7251): 113-7.
  • 7Karlsson O, Thor S, Norberg T, Ohlsson H, Edlund T. Insulin gene enhancer binding protein Isl- 1 is a member of a novel class of proteins containing both a borneo- and a Cys-His domain. Nature 1990, 344(6269): 879-82.
  • 8Fonoudi H, Yeganeh M, Fattahi F, Ghazizadeh Z, Rassouli H, Alikhani M, et al. ISL1 protein transduction promotes cardiomyocyte differentiation from human embryonic stem cells. PLoS One 2013, 8(1): e55577.
  • 9Ma Q, Zhou B, Pu WT. Reassessment of Isll and Nkx2-5 cardiac fate maps using a Gata4-based reporter of Cre activity. Dev Biol 2008, 323(1): 98-104.
  • 10Genead R, Danielsson C, Andersson AB, Corbascio M, Franco- Cereceda A, Sylven C, et al. Islet-1 cells are cardiac progenitors present during the entire lifespan: From the embryonic stage to adulthood. Stem Cells Dev 2010, 19(10): 1601-15.

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