硫酸盐还原菌去除木屑中木质素和纤维素的试验

Experimental Research on Removal of Ligno-Cellulose Using Sulfate-Reducing Bacteria

目的研究对不同木屑投加量利用硫酸盐还原菌降解木屑中木质素和纤维素去除率问题,为木质纤维素的生物降解提供新方法.方法将先期富集培养的硫酸盐还原菌污泥置于Ⅰ,Ⅱ,Ⅲ,Ⅳ4个三角瓶中,Ⅴ号三角瓶作为空白试验,分别投加5 g,10 g,15 g,20 g,10 g质量的木屑,通过静态试验测定木质纤维去除率.结果Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ号容器中木质素的去除率分别为49.32%,54.97%,57.92%,63.57%,0.07%;纤维素的去除率分别为82.18%,86.36%,89.32%,90.82%,7.33%.硫酸盐还原菌利用1 g的硫酸盐能够去除1 g的木质素.扫描电镜下观察经过硫酸盐还原菌降解后的木屑,发现木屑表面以及内部的结构均受到损伤,木质素和纤维素的结构被破坏.结论硫酸盐还原菌对木质纤维素有良好的降解作用.

The paper presented a new method for removal of ligno-cellulose biodegradation using sulfate reducing bacteria （ SRB ）. After cultivating SRB, the sludges were put into 4 erlenmeyer flasks named Ⅰ,Ⅱ,Ⅲ,Ⅳ4 and the V was taken as a blank test. These five reactors were filled by sawdusts with different dosages of 5 g,10 g,15 g,20 g and 10 g,respectively. The removal rates of ligno-cellulose were determined by the captive test. It is found that lignin removal rates are 49.32% ,54.97% ,57.92% ,63.57%, and 0.07%, respectively; and cellulose removal rates are 82. 18% ,86.36% ,89.32% ,90. 82%, and 7.33%, respectively. Sulfate reducing bacteria can remove I g lignin using 1 g sulphate. The surface and internal structure of sawdust are both damaged by SRB degradation under SEM, meanwhile the structure of ligno-cellulos is also destroyed. It indi-cates that SRB has good degradation effects on ligno-cellulose.