米碎花药效微小核糖核酸的鉴定及其靶基因功能分析

Identification of MicroRNAs from Eurya chinensis and function analysis on their target genes

目的筛选米碎花Eurya chinensis茎叶的微小核糖核酸(Micro RNAs,mi RNAs)类药效物质及其靶基因功能分析。方法采用高通量测序法,测定米碎花、正常小鼠和喂食米碎花匀浆液小鼠肝组织及血浆中mi RNAs的表达丰度,并与Gene Bank上的植物mi RNAs数据库比对,结合实时荧光定量PCR(RTFQ-PCR)验证,鉴定吸收进入体内的米碎花mi RNAs。以人类m RNA为靶标,采用Target Scan与Mi Randa预测米碎花mi RNAs的靶基因,并通过基因功能聚类(GO)与KEGG分析靶基因的相关功能。结果共鉴定出5个米碎花mi RNAs可吸收进入体内,其主要在生化过程、细胞成分和分子功能3大区域发挥作用,并影响肿瘤、代谢、炎症等相关信号通路。结论 mi RNAs可能是一类新型的中药药效物质。

Objective To investigate the pharmacodynamic Micro RNAs（miRNAs） in Eurya chinensis and the functions of their target genes. Methods The mi RNA profiles of E. chinensis in liver and plasma of normal mice and E. chinensis extract-treated mice were detected using high-flux sequencing, and then the miRNAs which took into the organism were validated by RTFQ-PCR using plant mi RNA database in Gene Bank. Human mRNA database as target was used to predict the target genes of miRNAs by Target Scan and Mi Randa analysis, while the target gene functions were considered by GO and KEGG analyses. Results Five pharmacodynamic micro RNAs were identified and took part in biological processes, cellular components, and molecular functions, which affected tumor, metabolism, and inflammation-related signal pathways. Conclusion The miRNAs might act as new pharmacodynamic materials of Chinese materia medica.